Literature DB >> 8702299

Linear correlation between bacterial overexpression of recombinant peptides and cell light scatter.

F Lavergne-Mazeau1, A Maftah, Y Cenatiempo, R Julien.   

Abstract

Fusion of multiple copies of a test peptide leads to insoluble inclusion bodies. Their presence within bacteria increases either forward-angle light scattering or, to a lesser extent, right-angle light scattering. A linear correlation has been established between cell forward-angle scattering and the level of overexpression of atrial natriuretic peptide. The correlation is valid only for unlysed cells and is protein product specific.

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Year:  1996        PMID： 8702299      PMCID： PMC168093          DOI： 10.1128/aem.62.8.3042-3046.1996

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  8 in total

Review 1.  Quantifying heterogeneity: flow cytometry of bacterial cultures.

Authors:  D B Kell; H M Ryder; A S Kaprelyants; H V Westerhoff
Journal:  Antonie Van Leeuwenhoek       Date:  1991 Oct-Nov       Impact factor: 2.271

2.  Production, analysis and bioactivity of recombinant vasoactive intestinal peptide analogs.

Authors:  J Raingeaud; F Lavergne; V Lelievre; J M Muller; R Julien; Y Cenatiempo
Journal:  Biochimie       Date:  1996       Impact factor: 4.079

3.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

4.  Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.

Authors:  H Schägger; G von Jagow
Journal:  Anal Biochem       Date:  1987-11-01       Impact factor: 3.365

Review 5.  The purification of eukaryotic polypeptides synthesized in Escherichia coli.

Authors:  F A Marston
Journal:  Biochem J       Date:  1986-11-15       Impact factor: 3.857

6.  High-level expression of alpha-human atrial natriuretic peptide from multiple joined genes in Escherichia coli.

Authors:  M Lennick; J R Haynes; S H Shen
Journal:  Gene       Date:  1987       Impact factor: 3.688

7.  A rapid and potent natriuretic response to intravenous injection of atrial myocardial extract in rats.

Authors:  A J de Bold; H B Borenstein; A T Veress; H Sonnenberg
Journal:  Life Sci       Date:  1981-01-05       Impact factor: 5.037

8.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205
  8 in total
  3 in total

1.  The application of multi-parameter flow cytometry to the study of recombinant Escherichia coli batch fermentation processes.

Authors:  Gareth Lewis; Ian W Taylor; Alvin W Nienow; Christopher J Hewitt
Journal:  J Ind Microbiol Biotechnol       Date:  2004-07-13       Impact factor: 3.346

2.  Two distinct states of Escherichia coli cells that overexpress recombinant heterogeneous β-galactosidase.

Authors:  Yun Zhao; Wei He; Wei-Feng Liu; Chun-Chun Liu; Li-Kui Feng; Lei Sun; Yong-Bin Yan; Hai-Ying Hang
Journal:  J Biol Chem       Date:  2012-02-02       Impact factor: 5.157

3.  Minireactor-based high-throughput temperature profiling for the optimization of microbial and enzymatic processes.

Authors:  Martin Kunze; Clemens Lattermann; Sylvia Diederichs; Wolfgang Kroutil; Jochen Büchs
Journal:  J Biol Eng       Date:  2014-08-04       Impact factor: 4.355
  3 in total

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