Literature DB >> 870047

The analysis of progress curves for enzyme-catalysed reactions by non-linear regression.

R G Duggleby, J F Morrison.   

Abstract

A procedure, based on the Gauss-Newton method for non-linear regression, has been developed to obtain enzyme kinetic constants from the analysis of progress curve data. Rules are presented which greatly simplify the derivation of the necessary equations. The method has been applied to the reactions catalysed by prephenate dehydratase, acid phosphatase and lactate dehydrogenase and has yielded values for kinetic parameters which agree well with those obtained from steady-state rate measurements.

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Year:  1977        PMID： 870047     DOI： 10.1016/0005-2744(77)90264-9

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  22 in total

1.  Exosite-mediated substrate recognition of factor IX by factor XIa. The factor XIa heavy chain is required for initial recognition of factor IX.

Authors:  Taketoshi Ogawa; Ingrid M Verhamme; Mao-Fu Sun; Paul E Bock; David Gailani
Journal:  J Biol Chem       Date:  2005-04-13       Impact factor: 5.157

2.  Deterministic three-half-order kinetic model for microbial degradation of added carbon substrates in soil.

Authors:  W Brunner; D D Focht
Journal:  Appl Environ Microbiol       Date:  1984-01       Impact factor: 4.792

3.  Rapid determination of enzyme kinetics from fluorescence: overcoming the inner filter effect.

Authors:  Mark O Palmier; Steven R Van Doren
Journal:  Anal Biochem       Date:  2007-07-18       Impact factor: 3.365

4.  Simultaneous estimation ofV max, K m, and the rate of endogenous substrate production (R) from substrate depletion data.

Authors:  J A Robinson; W G Characklis
Journal:  Microb Ecol       Date:  1984-06       Impact factor: 4.552

5.  Kinetics of hydrogen consumption by rumen fluid, anaerobic digestor sludge, and sediment.

Authors:  J A Robinson; J M Tiedje
Journal:  Appl Environ Microbiol       Date:  1982-12       Impact factor: 4.792

6.  Kinetic explanation for accumulation of nitrite, nitric oxide, and nitrous oxide during bacterial denitrification.

Authors:  M R Betlach; J M Tiedje
Journal:  Appl Environ Microbiol       Date:  1981-12       Impact factor: 4.792

7.  Mutational analysis of the active site of indoleglycerol phosphate synthase from Escherichia coli.

Authors:  B Darimont; C Stehlin; H Szadkowski; K Kirschner
Journal:  Protein Sci       Date:  1998-05       Impact factor: 6.725

8.  Analysis of progress curves for enzyme-catalysed reactions. Automatic construction of computer programs for fitting integrated rate equations.

Authors:  R G Duggleby; C Wood
Journal:  Biochem J       Date:  1989-03-01       Impact factor: 3.857

9.  Sucrose octasulfate selectively accelerates thrombin inactivation by heparin cofactor II.

Authors:  Suryakala Sarilla; Sally Y Habib; Dmitri V Kravtsov; Anton Matafonov; David Gailani; Ingrid M Verhamme
Journal:  J Biol Chem       Date:  2010-01-06       Impact factor: 5.157

10.  Fibrin monomer protects thrombin from inactivation by heparin-antithrombin III: implications for heparin efficacy.

Authors:  P J Hogg; C M Jackson
Journal:  Proc Natl Acad Sci U S A       Date:  1989-05       Impact factor: 11.205
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