Literature DB >> 869915

A novel system for the two-dimensional electrophoresis of membrane proteins.

A G Booth.   

Abstract

Membrane proteins were resolved in two dimensions by a novel technique that uses discontinuous electrophoresis in both directions. After electrophoresis in the first direction in chloral hydrate, the membrane proteins were further resolved by a novel system that used organic-base dodecyl sulphates to stack and then resolve them. This latter system has several advantages over conventional electrophoresis in sodium dodecyl sulphate, notably that it avoids the production of artifacts generated by other systems.

Entities:  

Mesh：

Substances：

Year:  1977        PMID： 869915      PMCID： PMC1164673          DOI： 10.1042/bj1630165

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  18 in total

1.  DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors:  B J DAVIS
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

2.  DISC ELECTROPHORESIS. I. BACKGROUND AND THEORY.

Authors:  L ORNSTEIN
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

3.  High resolution two-dimensional electrophoresis of proteins.

Authors:  P H O'Farrell
Journal:  J Biol Chem       Date:  1975-05-25       Impact factor: 5.157

4.  The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.

Authors:  J A Spudich; S Watt
Journal:  J Biol Chem       Date:  1971-08-10       Impact factor: 5.157

5.  A rapid method for the preparation of microvilli from rabbit kidney.

Authors:  A G Booth; A J Kenny
Journal:  Biochem J       Date:  1974-09       Impact factor: 3.857

6.  Multiphasic zone electrophoresis. II. Design of integrated discontinuous buffer systems for analytical and preparative fractionation.

Authors:  T M Jovin
Journal:  Biochemistry       Date:  1973-02-27       Impact factor: 3.162

7.  Multiphasic zone electrophoresis. 3. Further analysis and new forms of discontinuous buffer systems.

Authors:  T M Jovin
Journal:  Biochemistry       Date:  1973-02-27       Impact factor: 3.162

8.  Multiphasic zone electrophoresis. I. Steady-state moving-boundary systems formed by different electrolyte combinations.

Authors:  T M Jovin
Journal:  Biochemistry       Date:  1973-02-27       Impact factor: 3.162

9.  Dipeptidyl peptidase IV, a kidney brush-border serine peptidase.

Authors:  A J Kenny; A G Booth; S G George; J Ingram; D Kershaw; E J Wood; A R Young
Journal:  Biochem J       Date:  1976-07-01       Impact factor: 3.857

10.  Chloral hydrate: a solvent for biological membranes.

Authors:  B Ballou; G Sundharadas; M L Bach
Journal:  Science       Date:  1974-08-09       Impact factor: 47.728
View more
  8 in total

1.  Gel electrophoresis of the human erythrocyte membrane proteins: aberrant patterns in hematological and non-hematological diseases.

Authors:  V Anselstetter
Journal:  Blut       Date:  1978-03-15

2.  Proteins of the kidney microvillar membrane. Immunoelectrophoretic analysis of the membrane hydrolases: identification and resolution of the detergent- and proteinase-solubilized forms.

Authors:  A G Booth; L M Hubbard; A J Kenny
Journal:  Biochem J       Date:  1979-05-01       Impact factor: 3.857

3.  Proteins of the kidney microvillar membrane. The amphipathic form of dipeptidyl peptidase IV.

Authors:  D C Macnair; A J Kenny
Journal:  Biochem J       Date:  1979-05-01       Impact factor: 3.857

4.  Experimental pharmacological Parkinsonism (preliminary report).

Authors:  G Gomirato; H Hydén; E Mussini; L Rönnbäck
Journal:  Ital J Neurol Sci       Date:  1983-12

5.  Ion-exchange chromatography in the presence of the non-ionic dissociating agent chloral hydrate. Application to a membrane protein, bovine heart cytochrome c oxidase.

Authors:  D C Griffin; M Landon
Journal:  Biochem J       Date:  1982-01-01       Impact factor: 3.857

6.  Proteins of the kidney microvillar membrane. Aspartate aminopeptidase: purification by immunoadsorbent chromatography and properties of the detergent- and proteinase-solubilized forms.

Authors:  E M Danielsen; O Norén; H Sjöström; J Ingram; A J Kenny
Journal:  Biochem J       Date:  1980-09-01       Impact factor: 3.857

7.  Additional components of bovine heart cytochrome c oxidase demonstrated by high-resolution polyacrylamide-gel electrophoresis in the presence of chloral hydrate.

Authors:  D C Griffin; M Landon
Journal:  Biochem J       Date:  1981-08-01       Impact factor: 3.857

8.  Proteins of the kidney microvillar membrane. Asymmetric labelling of the membrane by lactoperoxidase-catalysed radioiodination and by photolysis of 3,5-di[125I]iodo-4-azidobenzenesulphonate.

Authors:  A G Booth; A J Kenny
Journal:  Biochem J       Date:  1980-04-01       Impact factor: 3.857
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.