Literature DB >> 4841584

Chloral hydrate: a solvent for biological membranes.

B Ballou, G Sundharadas, M L Bach.   

Abstract

A buffer system conitainin chloral hydrate, taurine, and bromopyridinium lactate was used to dissolve several biological membranes and separate their protein components by polyacrylamide gel electrophoresis. This solvent system was capable of separating molecules of similar size on the hasis of their charge and allows easy recovery of the proteins Thus. aqueous chloral hydrate is an effective solvent for biological membranes.

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Year:  1974        PMID: 4841584     DOI: 10.1126/science.185.4150.531

Source DB:  PubMed          Journal:  Science        ISSN: 0036-8075            Impact factor:   47.728


  2 in total

1.  A novel system for the two-dimensional electrophoresis of membrane proteins.

Authors:  A G Booth
Journal:  Biochem J       Date:  1977-04-01       Impact factor: 3.857

2.  Additional components of bovine heart cytochrome c oxidase demonstrated by high-resolution polyacrylamide-gel electrophoresis in the presence of chloral hydrate.

Authors:  D C Griffin; M Landon
Journal:  Biochem J       Date:  1981-08-01       Impact factor: 3.857

  2 in total

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