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Literature DB >> 8685264

Functional expression of the extraplastidial Arabidopsis thaliana oleate desaturase gene (FAD2) in Saccharomyces cerevisiae.

Abstract

The functional expression in yeast of the Arabidopsis thaliana FAD2 gene, encoding the extraplastidial oleate desaturase (1-acyl-2-oleoyl-sn-glycero-3-phosphocholine delta 12-desaturase) is reported. Dienoic fatty acids constituted up to 11% (w/w) of the total fatty acids in transformed Saccharomyces cerevisiae cells and were confirmed to be linoleic acid and delta 9, delta 12-hexadecadienoic acid by gas chromatography-mass spectrometry.

Entities: Chemical Gene Species

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Year: 1996 PMID： 8685264 PMCID： PMC157829 DOI： 10.1104/pp.111.1.223

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

12 in total

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10. Arabidopsis FAD2 gene encodes the enzyme that is essential for polyunsaturated lipid synthesis.

Authors: J Okuley; J Lightner; K Feldmann; N Yadav; E Lark; J Browse
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34 in total

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Authors: John M Dyer; Dorselyn C Chapital; Jui-Chang W Kuan; Robert T Mullen; Charlotta Turner; Thomas A McKeon; Armand B Pepperman
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6. Functional characterization of flax fatty acid desaturase FAD2 and FAD3 isoforms expressed in yeast reveals a broad diversity in activity.

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