Literature DB >> 1989527

The role of cytochrome b5 in delta 12 desaturation of oleic acid by microsomes of safflower (Carthamus tinctorius L.).

E V Kearns1, S Hugly, C R Somerville.   

Abstract

The electron donors for the membrane-bound fatty acid desaturases of higher plants have not previously been identified. In order to assess the participation of cytochrome b5 in microsomal fatty acid desaturation, the cytoplasmic domain of microsomal cytochrome b5 was purified from Brassica oleracea, and murine polyclonal antibodies were prepared. The IgG fraction from ascites fluid inhibited 62% of NADH-dependent cytochrome c reduction in safflower (Carthamus tinctorius L.) microsomes. These antibodies also blocked desaturation of oleic acid to linoleic acid in lipids of C. tinctorius microsomes by 93%, suggesting that cytochrome b5 is the electron donor for the delta 12 desaturase.

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Year:  1991        PMID： 1989527     DOI： 10.1016/0003-9861(91)90319-e

Source DB:  PubMed          Journal:  Arch Biochem Biophys        ISSN： 0003-9861            Impact factor:   4.013


  26 in total

1.  Cytochrome b5 reductase encoded by CBR1 is essential for a functional male gametophyte in Arabidopsis.

Authors:  Laura L Wayne; James G Wallis; Rajesh Kumar; Jonathan E Markham; John Browse
Journal:  Plant Cell       Date:  2013-08-30       Impact factor: 11.277

2.  Primary Structure of Cytochrome b(5) from Cauliflower (Brassica oleracea L.) Deduced from Peptide and cDNA Sequences.

Authors:  E V Kearns; P Keck; C R Somerville
Journal:  Plant Physiol       Date:  1992-07       Impact factor: 8.340

3.  Molecular and biochemical characterization of the OLE-1 high-oleic castor seed (Ricinus communis L.) mutant.

Authors:  Mónica Venegas-Calerón; Rosario Sánchez; Joaquín J Salas; Rafael Garcés; Enrique Martínez-Force
Journal:  Planta       Date:  2016-04-07       Impact factor: 4.116

4.  Microsomal electron transfer in higher plants: cloning and heterologous expression of NADH-cytochrome b5 reductase from Arabidopsis.

Authors:  M Fukuchi-Mizutani; M Mizutani; Y Tanaka; T Kusumi; D Ohta
Journal:  Plant Physiol       Date:  1999-01       Impact factor: 8.340

5.  A cytochrome b5 is required for full activity of flavonoid 3', 5'-hydroxylase, a cytochrome P450 involved in the formation of blue flower colors.

Authors:  N de Vetten; J ter Horst; H P van Schaik; A de Boer; J Mol; R Koes
Journal:  Proc Natl Acad Sci U S A       Date:  1999-01-19       Impact factor: 11.205

6.  Expression of a borage desaturase cDNA containing an N-terminal cytochrome b5 domain results in the accumulation of high levels of delta6-desaturated fatty acids in transgenic tobacco.

Authors:  O Sayanova; M A Smith; P Lapinskas; A K Stobart; G Dobson; W W Christie; P R Shewry; J A Napier
Journal:  Proc Natl Acad Sci U S A       Date:  1997-04-15       Impact factor: 11.205

7.  Temporal and spatial gene expression of cytochrome B5 during flower and fruit development in olives.

Authors:  A I Martsinkovskaya; Z P Poghosyan; K Haralampidis; D J Murphy; P Hatzopoulos
Journal:  Plant Mol Biol       Date:  1999-05       Impact factor: 4.076

8.  Changes in fatty acid composition in plant tissues expressing a mammalian delta9 desaturase.

Authors:  H Moon; J Hazebroek; D F Hildebrand
Journal:  Lipids       Date:  2000-05       Impact factor: 1.880

9.  Changes in electron transport pathways in endoplasmic reticulum of rapeseed in response to cold.

Authors:  Jacques Davy de Virville; Françoise Cochet; Guergana Tasseva; François Moreau; Alain Zachowski
Journal:  Planta       Date:  2008-07-29       Impact factor: 4.116

10.  Direct desaturation of intact galactolipids by a desaturase solubilized from spinach (Spinacia oleracea) chloroplast envelopes.

Authors:  H Schmidt; E Heinz
Journal:  Biochem J       Date:  1993-02-01       Impact factor: 3.857
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