Literature DB >> 8680936

Direct transformation of Neisseria gonorrhoeae by gel-isolated DNA.

K H Pritchard1, H S Seifert.   

Abstract

The naturally competent organism, Neisseria gonorrhoeae, can efficiently transform a marker carried on DNA purified in low-melting-temperature agarose without prior purification or dilution. Neither the agarose or buffer components inhibit transformation frequencies, but exposure to UV irradiation completely abrogates transformation.

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Year:  1995        PMID： 8680936     DOI： 10.1007/BF02779023

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  6 in total

1.  Cloning, restriction digestion and DNA labeling of large DNA fragments (greater than or equal to 1 kb) in the presence of remelted SeaPlaque GTG agarose gels.

Authors:  H A Daum; H W White; C M Seidell; P A Johnson
Journal:  Biotechniques       Date:  1991-12       Impact factor: 1.993

2.  Shuttle mutagenesis of Neisseria gonorrhoeae: pilin null mutations lower DNA transformation competence.

Authors:  H S Seifert; R S Ajioka; D Paruchuri; F Heffron; M So
Journal:  J Bacteriol       Date:  1990-01       Impact factor: 3.490

3.  Shuttle mutagenesis: two mini-transposons for gene mapping and for lacZ transcriptional fusions in Neisseria gonorrhoeae.

Authors:  S Boyle-Vavra; H S Seifert
Journal:  Gene       Date:  1993-07-15       Impact factor: 3.688

4.  Identification and arrangement of the DNA sequence recognized in specific transformation of Neisseria gonorrhoeae.

Authors:  S D Goodman; J J Scocca
Journal:  Proc Natl Acad Sci U S A       Date:  1988-09       Impact factor: 11.205

5.  NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION.

Authors:  D S KELLOGG; W L PEACOCK; W E DEACON; L BROWN; D I PIRKLE
Journal:  J Bacteriol       Date:  1963-06       Impact factor: 3.490

6.  Pilin expression in Neisseria gonorrhoeae is under both positive and negative transcriptional control.

Authors:  M K Taha; M So; H S Seifert; E Billyard; C Marchal
Journal:  EMBO J       Date:  1988-12-20       Impact factor: 11.598
  6 in total

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