Literature DB >> 2152910

Shuttle mutagenesis of Neisseria gonorrhoeae: pilin null mutations lower DNA transformation competence.

H S Seifert1, R S Ajioka, D Paruchuri, F Heffron, M So.   

Abstract

The method of shuttle mutagenesis has been extended to Neisseria gonorrhoeae. We have constructed a defective mini-Tn3 derivative that encodes chloramphenicol resistance in both N. gonorrhoeae and Escherichia coli and selected for mutations in the chloramphenicol resistance gene that express higher levels of antibiotic resistance in N. gonorrhoeae. Isogenic N. gonorrhoeae strains that differ only in pilin expression were constructed and used to test the effect of pilin null mutations on DNA transformation competence.

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Year:  1990        PMID： 2152910      PMCID： PMC208398          DOI： 10.1128/jb.172.1.40-46.1990

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  32 in total

1.  Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria.

Authors:  W V Shaw
Journal:  Methods Enzymol       Date:  1975       Impact factor: 1.600

2.  Specificity of DNA uptake in genetic transformation of gonococci.

Authors:  T J Dougherty; A Asmus; A Tomasz
Journal:  Biochem Biophys Res Commun       Date:  1979-01-15       Impact factor: 3.575

3.  Transduction versus "conjuduction": evidence for multiple roles for exonuclease V in genetic recombination in Escherichia coli.

Authors:  R D Porter; T McLaughlin; B Low
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1979

4.  Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.

Authors:  M Dagert; S D Ehrlich
Journal:  Gene       Date:  1979-05       Impact factor: 3.688

5.  Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors:  D B Clewell; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1969-04       Impact factor: 11.205

6.  Piliation control mechanisms in Neisseria gonorrhoeae.

Authors:  S Bergström; K Robbins; J M Koomey; J Swanson
Journal:  Proc Natl Acad Sci U S A       Date:  1986-06       Impact factor: 11.205

7.  Pilus expression in Neisseria gonorrhoeae involves chromosomal rearrangement.

Authors:  T F Meyer; N Mlawer; M So
Journal:  Cell       Date:  1982-08       Impact factor: 41.582

8.  A general method for site-directed mutagenesis in prokaryotes.

Authors:  G B Ruvkun; F M Ausubel
Journal:  Nature       Date:  1981-01-01       Impact factor: 49.962

9.  Genetic transformation of Neisseria gonorrhoeae to streptomycin resistance.

Authors:  P F Sparling
Journal:  J Bacteriol       Date:  1966-11       Impact factor: 3.490

10.  NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION.

Authors:  D S KELLOGG; W L PEACOCK; W E DEACON; L BROWN; D I PIRKLE
Journal:  J Bacteriol       Date:  1963-06       Impact factor: 3.490
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  58 in total

1.  Insertion mutations in pilE differentially alter gonococcal pilin antigenic variation.

Authors:  B Howell-Adams; H S Seifert
Journal:  J Bacteriol       Date:  1999-10       Impact factor: 3.490

2.  The meningococcal PilT protein is required for induction of intimate attachment to epithelial cells following pilus-mediated adhesion.

Authors:  C Pujol; E Eugène; M Marceau; X Nassif
Journal:  Proc Natl Acad Sci U S A       Date:  1999-03-30       Impact factor: 11.205

3.  A homologue of the recombination-dependent growth gene, rdgC, is involved in gonococcal pilin antigenic variation.

Authors:  I J Mehr; C D Long; C D Serkin; H S Seifert
Journal:  Genetics       Date:  2000-02       Impact factor: 4.562

4.  A mutant form of the Neisseria gonorrhoeae pilus secretin protein PilQ allows increased entry of heme and antimicrobial compounds.

Authors:  Ching-ju Chen; Deborah M Tobiason; Christopher E Thomas; William M Shafer; H Steven Seifert; P Frederick Sparling
Journal:  J Bacteriol       Date:  2004-02       Impact factor: 3.490

5.  Cloning and linkage analysis of Neisseria gonorrhoeae DNA methyltransferases.

Authors:  J S Gunn; A Piekarowicz; R Chien; D C Stein
Journal:  J Bacteriol       Date:  1992-09       Impact factor: 3.490

6.  Neisseria gonorrhoeae PilC expression provides a selective mechanism for structural diversity of pili.

Authors:  A B Jonsson; J Pfeifer; S Normark
Journal:  Proc Natl Acad Sci U S A       Date:  1992-04-15       Impact factor: 11.205

7.  Determination of surface-exposed, functional domains of gonococcal transferrin-binding protein A.

Authors:  Mary Kate Yost-Daljev; Cynthia Nau Cornelissen
Journal:  Infect Immun       Date:  2004-03       Impact factor: 3.441

8.  Genetic Manipulation of Neisseria gonorrhoeae.

Authors:  Joseph P Dillard
Journal:  Curr Protoc Microbiol       Date:  2011-11

9.  New complementation constructs for inducible and constitutive gene expression in Neisseria gonorrhoeae and Neisseria meningitidis.

Authors:  Meghan E Ramsey; Kathleen T Hackett; Chaitra Kotha; Joseph P Dillard
Journal:  Appl Environ Microbiol       Date:  2012-02-10       Impact factor: 4.792

10.  Identification of ZipA, a signal recognition particle-dependent protein from Neisseria gonorrhoeae.

Authors:  Ying Du; Cindy Grove Arvidson
Journal:  J Bacteriol       Date:  2003-04       Impact factor: 3.490
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