Literature DB >> 8680933

Determination of the molecular weight of DNA-binding proteins using UV-crosslinking and SDS-PAGE.

S S Wolf1, K Roder, M Schweizer.   

Abstract

We describe the use of UV-crosslinking in combination with SDS-PAGE to determine the approximate molecular weight of DNA-binding proteins. A 5-bromo-2'-deoxyuridine (5-BrdU)-substituted, radioactively labeled double-stranded oligonucleotide representing the protein binding site is incubated with a crude nuclear extract containing the protein of interest. Following irradiation with a UV light source, the DNA/protein complex is subjected to SDS-PAGE and its molecular weight determined by comparison with appropriate protein standards.

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Year:  1995        PMID： 8680933     DOI： 10.1007/BF02779020

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  11 in total

1.  The liver-specific transcription factor LF-B1 contains a highly diverged homeobox DNA binding domain.

Authors:  M Frain; G Swart; P Monaci; A Nicosia; S Stämpfli; R Frank; R Cortese
Journal:  Cell       Date:  1989-10-06       Impact factor: 41.582

2.  Photochemical cross-linking studies on the interaction of Escherichia coli RNA polymerase with T7 DNA.

Authors:  Z Hillel; C W Wu
Journal:  Biochemistry       Date:  1978-07-25       Impact factor: 3.162

3.  Purification and properties of Drosophila heat shock activator protein.

Authors:  C Wu; S Wilson; B Walker; I Dawid; T Paisley; V Zimarino; H Ueda
Journal:  Science       Date:  1987-11-27       Impact factor: 47.728

4.  Selective elimination of the exonuclease activity of the deoxyribonucleic acid polymerase from Escherichia coli B by limited proteolysis.

Authors:  H Klenow; I Henningsen
Journal:  Proc Natl Acad Sci U S A       Date:  1970-01       Impact factor: 11.205

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

Review 6.  The gel retardation assay.

Authors:  V Scott; A R Clark; K Docherty
Journal:  Methods Mol Biol       Date:  1994

7.  Characterization of the nuclear proteins binding the CACCC element of a glucocorticoid-responsive enhancer in the tyrosine aminotransferase gene.

Authors:  C DeVack; B Lupp; M Nichols; E Kowenz-Leutz; W Schmid; G Schütz
Journal:  Eur J Biochem       Date:  1993-02-01

8.  The application of 33P-labeling in the electrophoretic mobility shift assay.

Authors:  S S Wolf; J G Hopley; M Schweizer
Journal:  Biotechniques       Date:  1994-04       Impact factor: 1.993

9.  A mixed photoproduct of uracil and cysteine (5-S-cysteine-6-hydrouracil). A possible model for the in vivo cross-linking of deoxyribonucleic acid and protein by ultraviolet light.

Authors:  K C Smith; R T Aplin
Journal:  Biochemistry       Date:  1966-06       Impact factor: 3.162

10.  Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.

Authors:  J D Dignam; R M Lebovitz; R G Roeder
Journal:  Nucleic Acids Res       Date:  1983-03-11       Impact factor: 16.971
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