Literature DB >> 8679144

Repetitive element sequence-based PCR for species and strain discrimination in the genus Listeria.

B Jersek1, E Tcherneva, N Rijpens, L Herman.   

Abstract

Repetitive element sequence-based PCR (rep-PCR) was used to generate DNA fingerprints for Listeria spp. Two primer sets (REP 1R-I REP 2-I and ERIC 1R ERIC 2) used in respectively REP- and ERIC-PCR revealed that bacteria of the genus Listeria possess short repetitive extragenic palindromic elements and enterobacterial repetitive intergenic consensus sequences. Specific band profiles obtained by ERIC-PCR enabled the identification of Listeria species. With both REP- and ERIC-PCR the L. monocytogenes serotypes 1/2a, 1/2b, 1/2c, 3b and 4b could be clearly distinguished from each other. Within the serotype 1/2a, REP-PCR showed a higher discriminative potential than ERIC-PCR and a comparable discriminative potential as RAPD combining 3-4 primers.

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Year:  1996        PMID： 8679144     DOI： 10.1111/j.1472-765x.1996.tb00028.x

Source DB:  PubMed          Journal:  Lett Appl Microbiol        ISSN： 0266-8254            Impact factor:   2.858


  11 in total

1.  Absence of serotype-specific surface antigen and altered teichoic acid glycosylation among epidemic-associated strains of Listeria monocytogenes.

Authors:  E E Clark; I Wesley; F Fiedler; N Promadej; S Kathariou
Journal:  J Clin Microbiol       Date:  2000-10       Impact factor: 5.948

2.  A sheep in wolf's clothing: Listeria innocua strains with teichoic acid-associated surface antigens and genes characteristic of Listeria monocytogenes serogroup 4.

Authors:  Z Lan; F Fiedler; S Kathariou
Journal:  J Bacteriol       Date:  2000-11       Impact factor: 3.490

Review 3.  Methodologies for the characterization of microbes in industrial environments: a review.

Authors:  Johanna Maukonen; Jaana Mättö; Gun Wirtanen; Laura Raaska; Tiina Mattila-Sandholm; Maria Saarela
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Review 4.  Advantages and limitations of potential methods for the analysis of bacteria in milk: a review.

Authors:  Frederick Tawi Tabit
Journal:  J Food Sci Technol       Date:  2015-08-19       Impact factor: 2.701

5.  Specific identification of Listeria welshimeri and Listeria monocytogenes by PCR assays targeting a gene encoding a fibronectin-binding protein.

Authors:  Philippe Gilot; Jean Content
Journal:  J Clin Microbiol       Date:  2002-02       Impact factor: 5.948

6.  Direct identification in food samples of Listeria spp. and Listeria monocytogenes by molecular methods.

Authors:  Luca Cocolin; Kalliopi Rantsiou; Lucilla Iacumin; Carlo Cantoni; Giuseppe Comi
Journal:  Appl Environ Microbiol       Date:  2002-12       Impact factor: 4.792

7.  Listeria monocytogenes serotype identification by PCR.

Authors:  Monica K Borucki; Douglas R Call
Journal:  J Clin Microbiol       Date:  2003-12       Impact factor: 5.948

8.  Typing of Listeria monocytogenes strains by repetitive element sequence-based PCR.

Authors:  B Jersek; P Gilot; M Gubina; N Klun; J Mehle; E Tcherneva; N Rijpens; L Herman
Journal:  J Clin Microbiol       Date:  1999-01       Impact factor: 5.948

9.  Molecular subtyping of poultry-associated type A Clostridium perfringens isolates by repetitive-element PCR.

Authors:  G R Siragusa; M D Danyluk; K L Hiett; M G Wise; S E Craven
Journal:  J Clin Microbiol       Date:  2006-03       Impact factor: 5.948

10.  Rapid identification of Listeria species by using restriction fragment length polymorphism of PCR-amplified 23S rRNA gene fragments.

Authors:  Delphine Paillard; Véronique Dubois; Robert Duran; Fany Nathier; Catherine Guittet; Pierre Caumette; Claudine Quentin
Journal:  Appl Environ Microbiol       Date:  2003-11       Impact factor: 4.792
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