Literature DB >> 8669898

Inactivation of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824.

E M Green1, G N Bennett.   

Abstract

A nonreplicative plasmid containing an internal aad gene fragment has been integrated into the chromosome of Clostridium acetobutylicum ATCC 824. Transformation was accomplished by electroporation with relatively high concentrations of methylated plasmid DNA. Southern hybridization experiments revealed that integration occurred by single crossover homologous recombination inactivating the aad gene. Integrants were relatively stable after 25 generations. Inactivation of the aad gene drastically reduced solvent production. This result suggests that aldehyde/alcohol dehydrogenase(AAD) plays a important role in butanol production.

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Year:  1996        PMID： 8669898     DOI： 10.1007/bf02941702

Source DB:  PubMed          Journal:  Appl Biochem Biotechnol        ISSN： 0273-2289            Impact factor:   2.926


  20 in total

1.  Molecular characterization of two Clostridium acetobutylicum ATCC 824 butanol dehydrogenase isozyme genes.

Authors:  K A Walter; G N Bennett; E T Papoutsakis
Journal:  J Bacteriol       Date:  1992-11       Impact factor: 3.490

2.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

Review 3.  Recent advances in the genetics of the clostridia.

Authors:  M Young; N P Minton; W L Staudenbauer
Journal:  FEMS Microbiol Rev       Date:  1989-12       Impact factor: 16.408

4.  Conjugative plasmid transfer from Escherichia coli to Clostridium acetobutylicum.

Authors:  D R Williams; D I Young; M Young
Journal:  J Gen Microbiol       Date:  1990-05

5.  Stability of reiterated sequences in the Bacillus subtilis chromosome.

Authors:  M Young; S D Ehrlich
Journal:  J Bacteriol       Date:  1989-05       Impact factor: 3.490

6.  Campbell-like integration of heterologous plasmid DNA into the chromosome of Lactococcus lactis subsp. lactis.

Authors:  K J Leenhouts; J Kok; G Venema
Journal:  Appl Environ Microbiol       Date:  1989-02       Impact factor: 4.792

7.  Sequence and arrangement of genes encoding enzymes of the acetone-production pathway of Clostridium acetobutylicum ATCC824.

Authors:  D J Petersen; J W Cary; J Vanderleyden; G N Bennett
Journal:  Gene       Date:  1993-01-15       Impact factor: 3.688

8.  Plasmid replication stimulates DNA recombination in Bacillus subtilis.

Authors:  P Noirot; M A Petit; S D Ehrlich
Journal:  J Mol Biol       Date:  1987-07-05       Impact factor: 5.469

9.  Expression of plasmid-encoded aad in Clostridium acetobutylicum M5 restores vigorous butanol production.

Authors:  R V Nair; E T Papoutsakis
Journal:  J Bacteriol       Date:  1994-09       Impact factor: 3.490

10.  Cloning, sequencing, and molecular analysis of the sol operon of Clostridium acetobutylicum, a chromosomal locus involved in solventogenesis.

Authors:  R J Fischer; J Helms; P Dürre
Journal:  J Bacteriol       Date:  1993-11       Impact factor: 3.490
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  15 in total

Review 1.  Microbial cellulose utilization: fundamentals and biotechnology.

Authors:  Lee R Lynd; Paul J Weimer; Willem H van Zyl; Isak S Pretorius
Journal:  Microbiol Mol Biol Rev       Date:  2002-09       Impact factor: 11.056

Review 2.  Problems with the microbial production of butanol.

Authors:  Yan-Ning Zheng; Liang-Zhi Li; Mo Xian; Yu-Jiu Ma; Jian-Ming Yang; Xin Xu; Dong-Zhi He
Journal:  J Ind Microbiol Biotechnol       Date:  2009-06-27       Impact factor: 3.346

3.  2,4,6-trinitrotoluene reduction by an Fe-only hydrogenase in Clostridium acetobutylicum.

Authors:  Mary M Watrous; Sandra Clark; Razia Kutty; Shouqin Huang; Frederick B Rudolph; Joseph B Hughes; George N Bennett
Journal:  Appl Environ Microbiol       Date:  2003-03       Impact factor: 4.792

4.  Inactivation of σF in Clostridium acetobutylicum ATCC 824 blocks sporulation prior to asymmetric division and abolishes σE and σG protein expression but does not block solvent formation.

Authors:  Shawn W Jones; Bryan P Tracy; Stefan M Gaida; Eleftherios T Papoutsakis
Journal:  J Bacteriol       Date:  2011-03-18       Impact factor: 3.490

5.  Cloning, sequencing, and expression of genes encoding phosphotransacetylase and acetate kinase from Clostridium acetobutylicum ATCC 824.

Authors:  Z L Boynton; G N Bennett; F B Rudolph
Journal:  Appl Environ Microbiol       Date:  1996-08       Impact factor: 4.792

6.  Antisense RNA strategies for metabolic engineering of Clostridium acetobutylicum.

Authors:  R P Desai; E T Papoutsakis
Journal:  Appl Environ Microbiol       Date:  1999-03       Impact factor: 4.792

7.  A genetic system for Clostridium ljungdahlii: a chassis for autotrophic production of biocommodities and a model homoacetogen.

Authors:  Ching Leang; Toshiyuki Ueki; Kelly P Nevin; Derek R Lovley
Journal:  Appl Environ Microbiol       Date:  2012-11-30       Impact factor: 4.792

8.  Novel system for efficient isolation of Clostridium double-crossover allelic exchange mutants enabling markerless chromosomal gene deletions and DNA integration.

Authors:  Mohab A Al-Hinai; Alan G Fast; Eleftherios T Papoutsakis
Journal:  Appl Environ Microbiol       Date:  2012-09-14       Impact factor: 4.792

9.  Group II intron-anchored gene deletion in Clostridium.

Authors:  Kaizhi Jia; Yan Zhu; Yanping Zhang; Yin Li
Journal:  PLoS One       Date:  2011-01-31       Impact factor: 3.240

10.  Engineering clostridium strain to accept unmethylated DNA.

Authors:  Hongjun Dong; Yanping Zhang; Zongjie Dai; Yin Li
Journal:  PLoS One       Date:  2010-02-09       Impact factor: 3.240
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