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Literature DB >> 8657565

The small subunit of the splicing factor U2AF is conserved in fission yeast.

Abstract

The human splicing factor U2 auxiliary factor (hsU2AF) is comprised of two interacting subunits of 65 and 35 kDa. Previously we identified the Schizosaccharomyces pombe homolog, spU2AF59, of the human large subunit. We have screened a fission yeast cDNA library in search of proteins that interact with spU2AF59 using the yeast two-hybrid system and have identified a homolog of the hsU2AF35 subunit. The S. pombe U2AF small subunit is a single copy gene that encodes a protein which shares 55% amino acid identity and 17% similarity with the human small subunit. Unlike the human protein, the yeast protein lacks an arginine/serine-rich region. The predicted molecular mass of the spU2AF small subunit is 23 kDa. The region of spU2AF59 that interacts with spU2AF23 is similar to the region in which the human small and large subunits interact.

Entities: Chemical Gene Species

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Year: 1996 PMID： 8657565 PMCID： PMC145878 DOI： 10.1093/nar/24.10.1849

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

32 in total

1. U1 snRNP targets an essential splicing factor, U2AF65, to the 3' splice site by a network of interactions spanning the exon.

Authors: B E Hoffman; P J Grabowski
Journal: Genes Dev Date: 1992-12 Impact factor: 11.361

2. Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit.

Authors: M Zhang; P D Zamore; M Carmo-Fonseca; A I Lamond; M R Green
Journal: Proc Natl Acad Sci U S A Date: 1992-09-15 Impact factor: 11.205

3. Cloning and domain structure of the mammalian splicing factor U2AF.

Authors: P D Zamore; J G Patton; M R Green
Journal: Nature Date: 1992-02-13 Impact factor: 49.962

4. Arginine/serine-rich domains of the su(wa) and tra RNA processing regulators target proteins to a subnuclear compartment implicated in splicing.

Authors: H Li; P M Bingham
Journal: Cell Date: 1991-10-18 Impact factor: 41.582

Review 5. Biochemical mechanisms of constitutive and regulated pre-mRNA splicing.

Authors: M R Green
Journal: Annu Rev Cell Biol Date: 1991

6. Use of the two-hybrid system to identify the domain of p53 involved in oligomerization.

Authors: K Iwabuchi; B Li; P Bartel; S Fields
Journal: Oncogene Date: 1993-06 Impact factor: 9.867

7. U2AF homolog required for splicing in vivo.

Authors: J Potashkin; K Naik; K Wentz-Hunter
Journal: Science Date: 1993-10-22 Impact factor: 47.728

8. The conserved pre-mRNA splicing factor U2AF from Drosophila: requirement for viability.

Authors: R Kanaar; S E Roche; E L Beall; M R Green; D C Rio
Journal: Science Date: 1993-10-22 Impact factor: 47.728

9. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases.

Authors: J W Harper; G R Adami; N Wei; K Keyomarsi; S J Elledge
Journal: Cell Date: 1993-11-19 Impact factor: 41.582

10. Biochemical characterization of U2 snRNP auxiliary factor: an essential pre-mRNA splicing factor with a novel intranuclear distribution.

Authors: P D Zamore; M R Green
Journal: EMBO J Date: 1991-01 Impact factor: 11.598

27 in total

1. PUF60: a novel U2AF65-related splicing activity.

Authors: P S Page-McCaw; K Amonlirdviman; P A Sharp
Journal: RNA Date: 1999-12 Impact factor: 4.942

2. Mutation in the prp12+ gene encoding a homolog of SAP130/SF3b130 causes differential inhibition of pre-mRNA splicing and arrest of cell-cycle progression in Schizosaccharomyces pombe.

Authors: Y Habara; S Urushiyama; T Shibuya; Y Ohshima; T Tani
Journal: RNA Date: 2001-05 Impact factor: 4.942

The small subunit of the splicing factor U2AF is conserved in fission yeast.

1. U1 snRNP targets an essential splicing factor, U2AF65, to the 3' splice site by a network of interactions spanning the exon.

2. Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit.

3. Cloning and domain structure of the mammalian splicing factor U2AF.

4. Arginine/serine-rich domains of the su(wa) and tra RNA processing regulators target proteins to a subnuclear compartment implicated in splicing.

Review 5. Biochemical mechanisms of constitutive and regulated pre-mRNA splicing.

6. Use of the two-hybrid system to identify the domain of p53 involved in oligomerization.

7. U2AF homolog required for splicing in vivo.

8. The conserved pre-mRNA splicing factor U2AF from Drosophila: requirement for viability.

9. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases.

10. Biochemical characterization of U2 snRNP auxiliary factor: an essential pre-mRNA splicing factor with a novel intranuclear distribution.

1. PUF60: a novel U2AF65-related splicing activity.

2. Mutation in the prp12+ gene encoding a homolog of SAP130/SF3b130 causes differential inhibition of pre-mRNA splicing and arrest of cell-cycle progression in Schizosaccharomyces pombe.

Review 3. Analysis of the splicing machinery in fission yeast: a comparison with budding yeast and mammals.

4. Evidence for splice site pairing via intron definition in Schizosaccharomyces pombe.

Review 5. U2AF homology motifs: protein recognition in the RRM world.

6. A potential role for U2AF-SAP 155 interactions in recruiting U2 snRNP to the branch site.

7. Biochemical and genetic conservation of fission yeast Dsk1 and human SR protein-specific kinase 1.

8. Characterization of U2AF(6), a splicing factor related to U2AF(35).

9. U2AF35 is encoded by an essential gene clustered in an operon with RRM/cyclophilin in Caenorhabditis elegans.

10. Introns and splicing elements of five diverse fungi.