Literature DB >> 10606266

PUF60: a novel U2AF65-related splicing activity.

P S Page-McCaw1, K Amonlirdviman, P A Sharp.   

Abstract

We have identified a new pyrimidine-tract binding factor, PUF, that is required, together with U2AF, for efficient reconstitution of RNA splicing in vitro. The activity has been purified and consists of two proteins, PUF60 and the previously described splicing factor p54. p54 and PUF60 form a stable complex in vitro when cotranslated in a reaction mixture. PUF activity, in conjunction with U2AF, facilitates the association of U2 snRNP with the pre-mRNA. This reaction is dependent upon the presence of the large subunit of U2AF, U2AF65, but not the small subunit U2AF35. PUF60 is homologous to both U2AF65 and the yeast splicing factor Mud2p. The C-terminal domain of PUF60, the PUMP domain, is distantly related to the RNA-recognition motif domain, and is probably important in protein-protein interactions.

Entities:  

Mesh：

Substances：

Year:  1999        PMID： 10606266      PMCID： PMC1369877          DOI： 10.1017/s1355838299991938

Source DB:  PubMed          Journal:  RNA        ISSN： 1355-8382            Impact factor:   4.942


  45 in total

1.  Targeted snRNP depletion reveals an additional role for mammalian U1 snRNP in spliceosome assembly.

Authors:  S M Barabino; B J Blencowe; U Ryder; B S Sproat; A I Lamond
Journal:  Cell       Date:  1990-10-19       Impact factor: 41.582

2.  Identification, purification, and biochemical characterization of U2 small nuclear ribonucleoprotein auxiliary factor.

Authors:  P D Zamore; M R Green
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

3.  Separation and properties of cellular and scrapie prion proteins.

Authors:  R K Meyer; M P McKinley; K A Bowman; M B Braunfeld; R A Barry; S B Prusiner
Journal:  Proc Natl Acad Sci U S A       Date:  1986-04       Impact factor: 11.205

4.  Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs.

Authors:  M M Konarska; P A Sharp
Journal:  Cell       Date:  1986-09-12       Impact factor: 41.582

5.  A factor, U2AF, is required for U2 snRNP binding and splicing complex assembly.

Authors:  B Ruskin; P D Zamore; M R Green
Journal:  Cell       Date:  1988-01-29       Impact factor: 41.582

6.  Messenger RNA splicing in vitro: an excised intervening sequence and a potential intermediate.

Authors:  P J Grabowski; R A Padgett; P A Sharp
Journal:  Cell       Date:  1984-06       Impact factor: 41.582

7.  Cloning of Caenorhabditis U2AF65: an alternatively spliced RNA containing a novel exon.

Authors:  D A Zorio; K Lea; T Blumenthal
Journal:  Mol Cell Biol       Date:  1997-02       Impact factor: 4.272

8.  Maturation of the tail spike endorhamnosidase of Salmonella phage P22.

Authors:  D P Goldenberg; P B Berget; J King
Journal:  J Biol Chem       Date:  1982-07-10       Impact factor: 5.157

9.  Primary structure of a human arginine-rich nuclear protein that colocalizes with spliceosome components.

Authors:  N Chaudhary; C McMahon; G Blobel
Journal:  Proc Natl Acad Sci U S A       Date:  1991-09-15       Impact factor: 11.205

10.  Biochemical characterization of U2 snRNP auxiliary factor: an essential pre-mRNA splicing factor with a novel intranuclear distribution.

Authors:  P D Zamore; M R Green
Journal:  EMBO J       Date:  1991-01       Impact factor: 11.598
View more
  62 in total

1.  Functional selection of splicing enhancers that stimulate trans-splicing in vitro.

Authors:  L A Boukis; J P Bruzik
Journal:  RNA       Date:  2001-06       Impact factor: 4.942

Review 2.  U2AF homology motifs: protein recognition in the RRM world.

Authors:  Clara L Kielkopf; Stephan Lücke; Michael R Green
Journal:  Genes Dev       Date:  2004-07-01       Impact factor: 11.361

3.  Identification of alternative splicing regulators by RNA interference in Drosophila.

Authors:  Jung W Park; Katherine Parisky; Alicia M Celotto; Robert A Reenan; Brenton R Graveley
Journal:  Proc Natl Acad Sci U S A       Date:  2004-10-18       Impact factor: 11.205

Review 4.  A subset of nuclear receptor coregulators act as coupling proteins during synthesis and maturation of RNA transcripts.

Authors:  Didier Auboeuf; Dennis H Dowhan; Martin Dutertre; Natalia Martin; Susan M Berget; Bert W O'Malley
Journal:  Mol Cell Biol       Date:  2005-07       Impact factor: 4.272

5.  SRp54 (SFRS11), a regulator for tau exon 10 alternative splicing identified by an expression cloning strategy.

Authors:  Jane Y Wu; Amar Kar; David Kuo; Bing Yu; Necat Havlioglu
Journal:  Mol Cell Biol       Date:  2006-09       Impact factor: 4.272

6.  Multiple U2AF65 binding sites within SF3b155: thermodynamic and spectroscopic characterization of protein-protein interactions among pre-mRNA splicing factors.

Authors:  Karen R Thickman; Matthew C Swenson; Joseph M Kabogo; Zygmunt Gryczynski; Clara L Kielkopf
Journal:  J Mol Biol       Date:  2005-12-07       Impact factor: 5.469

7.  Genomic functions of U2AF in constitutive and regulated splicing.

Authors:  Tongbin Wu; Xiang-Dong Fu
Journal:  RNA Biol       Date:  2015       Impact factor: 4.652

8.  Sequence, Structure, and Context Preferences of Human RNA Binding Proteins.

Authors:  Daniel Dominguez; Peter Freese; Maria S Alexis; Amanda Su; Myles Hochman; Tsultrim Palden; Cassandra Bazile; Nicole J Lambert; Eric L Van Nostrand; Gabriel A Pratt; Gene W Yeo; Brenton R Graveley; Christopher B Burge
Journal:  Mol Cell       Date:  2018-06-07       Impact factor: 17.970

9.  Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein.

Authors:  Cindy L Will; Henning Urlaub; Tilmann Achsel; Marc Gentzel; Matthias Wilm; Reinhard Lührmann
Journal:  EMBO J       Date:  2002-09-16       Impact factor: 11.598

10.  Dual function for U2AF(35) in AG-dependent pre-mRNA splicing.

Authors:  S Guth; T Ø Tange; E Kellenberger; J Valcárcel
Journal:  Mol Cell Biol       Date:  2001-11       Impact factor: 4.272
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.