Literature DB >> 8652878

Direct database searching with MALDI-PSD spectra of peptides.

P R Griffin1, M J MacCoss, J K Eng, R A Blevins, J S Aaronson, J R Yates.   

Abstract

The analysis of matrix-assisted laser desorption ionization post-source decay (MALDI-PSD) mass spectra of peptides by using the cross-correlation method for database searching is illustrated. MALDI-PSD mass spectra are shown to contain sufficient fragmentation information to uniquely identify the correct amino acid sequence from large protein databases (approximately 160,000 entries). A search employing the MALDI-PSD mass spectrum of a phosphorylated peptide that correctly identifies the amino acid sequence and the site of phosphorylation is also illustrated.

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Year:  1995        PMID： 8652878     DOI： 10.1002/rcm.1290091515

Source DB:  PubMed          Journal:  Rapid Commun Mass Spectrom        ISSN： 0951-4198            Impact factor:   2.419


  8 in total

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  8 in total

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