Literature DB >> 86495

Detection of mycoplasma contamination in tissue cultures by fluorescence microscopy.

M Thangavelu, H Ernø, A J Therkelsen.   

Abstract

The in situ staining method of Chen (1977) for the detection of mycoplasma contaminants in tissue cultures was tested in cultures of human skin fibroblasts after controlled contamination with Mycoplasma arginini. It is concluded that this method is reliable only at infection rates of 100% or higher, i.e., at one mycoplasma or more per tissue-culture cell.

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Year:  1979        PMID: 86495     DOI: 10.1007/BF00273202

Source DB:  PubMed          Journal:  Hum Genet        ISSN: 0340-6717            Impact factor:   4.132


  6 in total

1.  Appearance of hypoxanthine guanine phosphoribosyltransferase activity as a consequence of mycoplasma contamination.

Authors:  E J Stanbridge; J A Tischfield; E L Schneider
Journal:  Nature       Date:  1975-07-24       Impact factor: 49.962

2.  Bovine mycoplasmas: cultural and biochemical studies. I.

Authors:  H Erno; L Stipkovits
Journal:  Acta Vet Scand       Date:  1973       Impact factor: 1.695

3.  Endogenous HPRT activity in a cryptic strain of mycoplasma and its effect on cellular resistance to selective media in infected cell lines.

Authors:  O P van Diggelen; D M Phillips; S I Shin
Journal:  Exp Cell Res       Date:  1977-04       Impact factor: 3.905

4.  In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain.

Authors:  T R Chen
Journal:  Exp Cell Res       Date:  1977-02       Impact factor: 3.905

5.  A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses.

Authors:  W C Russell; C Newman; D H Williamson
Journal:  Nature       Date:  1975-02-06       Impact factor: 49.962

6.  Mycoplasma contamination of cultured amniotic fluid cells: potential hazard to prenatal chromosomal diagnosis.

Authors:  E L Schneider; E J Stanbridge; C J Epstein; M Golbus; G Abbo-Halbasch; G Rodgers
Journal:  Science       Date:  1974-04-26       Impact factor: 47.728

  6 in total
  1 in total

1.  Species-specific PCR for identification of common contaminant mollicutes in cell culture.

Authors:  F Kong; G James; S Gordon; A Zelynski; G L Gilbert
Journal:  Appl Environ Microbiol       Date:  2001-07       Impact factor: 4.792

  1 in total

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