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Literature DB >> 8643553

Quantal acetylcholine release induced by mediatophore transfection.

J Falk-Vairant¹, P Corrèges, L Eder-Colli, N Salem, E Roulet, A Bloc, F Meunier, B Lesbats, F Loctin, M Synguelakis, M Israel, Y Dunant.

Abstract

Mediatophore is a protein of approximately 200 kDa able to translocate acetylcholine in response to calcium. It was purified from the presynaptic plasma membranes of the electric organ nerve terminals. Mediatophore is a homooligomer of a 16-kDa subunit, homologous to the proteolipid of V-ATPase. Cells of the N18TG-2 neuronal line are not able to produce quantal acetylcholine release. We show here that transfection of N18TG-2 cells with a plasmid encoding the mediatophore subunit restored calcium-dependent release. The essential feature of such a release was its quantal nature, similar to what is observed in situ in cholinergic synapses from which mediatophore was purified.

Entities: Chemical Gene

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Year: 1996 PMID： 8643553 PMCID： PMC39222 DOI： 10.1073/pnas.93.11.5203

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

23 in total

Review 1. Organellar proton-ATPases.

Authors: N Nelson
Journal: Curr Opin Cell Biol Date: 1992-08 Impact factor: 8.382

2. Acetylcholine receptor channels in Xenopus myocyte culture; brief openings, brief closures and slow desensitization.

Authors: Y Kidokoro; J Rohrbough
Journal: J Physiol Date: 1990-06 Impact factor: 5.182

Review 3. The E5 oncoprotein target: a 16-kDa channel-forming protein with diverse functions.

Authors: M E Finbow; J D Pitts; D J Goldstein; R Schlegel; J B Findlay
Journal: Mol Carcinog Date: 1991 Impact factor: 4.784

4. Purification of a presynaptic membrane protein that mediates a calcium-dependent translocation of acetylcholine.

Authors: M Israël; N Morel; B Lesbats; S Birman; R Manaranche
Journal: Proc Natl Acad Sci U S A Date: 1986-12 Impact factor: 11.205

5. Coregulation of two embedded gene products, choline acetyltransferase and the vesicular acetylcholine transporter.

Authors: S Berrard; H Varoqui; R Cervini; M Israël; J Mallet; M F Diebler
Journal: J Neurochem Date: 1995-08 Impact factor: 5.372

Review 6. Acetylcholine release, from molecules to function.

Authors: M Israël; Y Dunant
Journal: Prog Brain Res Date: 1993 Impact factor: 2.453

7. A 15 kDa proteolipid found in mediatophore preparations from Torpedo electric organ presents high sequence homology with the bovine chromaffin granule protonophore.

Authors: S Birman; F M Meunier; B Lesbats; J P Le Caer; J Rossier; M Israël
Journal: FEBS Lett Date: 1990-02-26 Impact factor: 4.124

8. Pulsatile release of acetylcholine by nerve terminals (synaptosomes) isolated from Torpedo electric organ.

Authors: R Girod; L Eder-Colli; J Medilanski; Y Dunant; N Tabti; M M Poo
Journal: J Physiol Date: 1992-05 Impact factor: 5.182

9. Immunolabelling of the presynaptic membrane of Torpedo electric organ nerve terminals with an antiserum towards the acetylcholine releasing protein mediatophore.

Authors: G Brochier; M Israël; B Lesbats
Journal: Biol Cell Date: 1993 Impact factor: 4.458

10. Release of acetylcholine by Xenopus oocytes injected with mRNAs from cholinergic neurons.

Authors: A Cavalli; L Eder-Colli; Y Dunant; F Loctin; N Morel
Journal: EMBO J Date: 1991-07 Impact factor: 11.598

14 in total

Review 1. Low- and high-affinity reactions in rapid neurotransmission.

Authors: Yves Dunant; Alain Bloc
Journal: Neurochem Res Date: 2003-04 Impact factor: 3.996

2. Acetylcholine release in rapid synapses: two fast partners--mediatophore and vesicular Ca2+/H+ antiport.

Authors: Yves Dunant
Journal: J Mol Neurosci Date: 2006 Impact factor: 3.444

3. The vesicular acetylcholine transporter is required for neuromuscular development and function.

Authors: Braulio M de Castro; Xavier De Jaeger; Cristina Martins-Silva; Ricardo D F Lima; Ernani Amaral; Cristiane Menezes; Patricia Lima; Cintia M L Neves; Rita G Pires; Thomas W Gould; Ian Welch; Christopher Kushmerick; Cristina Guatimosim; Ivan Izquierdo; Martin Cammarota; R Jane Rylett; Marcus V Gomez; Marc G Caron; Ronald W Oppenheim; Marco A M Prado; Vania F Prado
Journal: Mol Cell Biol Date: 2009-07-27 Impact factor: 4.272

Quantal acetylcholine release induced by mediatophore transfection.

Review 1. Organellar proton-ATPases.

2. Acetylcholine receptor channels in Xenopus myocyte culture; brief openings, brief closures and slow desensitization.

Review 3. The E5 oncoprotein target: a 16-kDa channel-forming protein with diverse functions.

4. Purification of a presynaptic membrane protein that mediates a calcium-dependent translocation of acetylcholine.

5. Coregulation of two embedded gene products, choline acetyltransferase and the vesicular acetylcholine transporter.

Review 6. Acetylcholine release, from molecules to function.

7. A 15 kDa proteolipid found in mediatophore preparations from Torpedo electric organ presents high sequence homology with the bovine chromaffin granule protonophore.

8. Pulsatile release of acetylcholine by nerve terminals (synaptosomes) isolated from Torpedo electric organ.

9. Immunolabelling of the presynaptic membrane of Torpedo electric organ nerve terminals with an antiserum towards the acetylcholine releasing protein mediatophore.

10. Release of acetylcholine by Xenopus oocytes injected with mRNAs from cholinergic neurons.

Review 1. Low- and high-affinity reactions in rapid neurotransmission.

2. Acetylcholine release in rapid synapses: two fast partners--mediatophore and vesicular Ca2+/H+ antiport.

3. The vesicular acetylcholine transporter is required for neuromuscular development and function.

Review 4. Ultra-fast versus sustained cholinergic transmission: a variety of different mechanisms.

Review 5. Acetylcholine release and the cholinergic genomic locus.

Review 6. In vitro reconstitution of neurotransmitter release.

Review 7. The vacuolar H+-ATPase: a universal proton pump of eukaryotes.

Review 8. The membrane domain of vacuolar H(+)ATPase: a crucial player in neurotransmitter exocytotic release.

9. Synaptic vesicles control the time course of neurotransmitter secretion via a Ca²+/H+ antiport.

10. Constitutive secretion of exogenous neurotransmitter by nonneuronal cells: implications for neuronal secretion.