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Literature DB >> 3466183

Purification of a presynaptic membrane protein that mediates a calcium-dependent translocation of acetylcholine.

M Israël, N Morel, B Lesbats, S Birman, R Manaranche.

Abstract

A protein, which we call "mediatophore," that mediates calcium-dependent release of acetylcholine from proteoliposomes has been purified from the presynaptic plasma membrane. About 250 micrograms of this material was obtained from 500 g of Torpedo marmorata electric organ. Precipitation of the protein and subsequent removal of associated lipids inactivated the protein, which then became water soluble; this permitted evaluation of its Stokes radius (52 A) and its sedimentation coefficient (9.8 +/- 0.75 S) and, hence, an approximate molecular mass of 210 +/- 16 kDa could be determined. PAGE analysis showed that the protein is made of 17-kDa subunits, not linked by disulfide bonds. When this material was observed by electron microscopy after negative staining, the apparently pentameric structures had an average diameter of about 7 nm.

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Year: 1986 PMID： 3466183 PMCID： PMC387108 DOI： 10.1073/pnas.83.23.9226

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

31 in total

1. Transmitter leakage from motor nerve endings.

Authors: B Katz; R Miledi
Journal: Proc R Soc Lond B Biol Sci Date: 1977-02-11

2. Isolation of a chloroplast N,N'-dicyclohexylcarbodiimide-binding proteolipid, active in proton translocation.

Authors: N Nelson; E Eytan; B E Notsani; H Sigrist; K Sigrist-Nelson; C Gitler
Journal: Proc Natl Acad Sci U S A Date: 1977-06 Impact factor: 11.205

3. A new and rapid colorimetric determination of acetylcholinesterase activity.

Authors: G L ELLMAN; K D COURTNEY; V ANDRES; R M FEATHER-STONE
Journal: Biochem Pharmacol Date: 1961-07 Impact factor: 5.858

4. [Changes in acetylcholine level and electrophysiological response during continuous stimulation of the electric organ of Torpedo marmorata (author's transl)].

Authors: Y Dunant; J Gautron; M Israël; B Lesbats; R Manaranche
Journal: J Neurochem Date: 1974-10 Impact factor: 5.372

Review 5. The present status of the vesicular hypothesis.

Authors: M Israel; Y Dunant; R Manaranche
Journal: Prog Neurobiol Date: 1979 Impact factor: 11.685

6. Proteolipids from membrane systems.

Authors: J Folch-Pi; P J Stoffyn
Journal: Ann N Y Acad Sci Date: 1972-06-20 Impact factor: 5.691

7. Selective solubilization of proteins from red blood cell membranes by protein perturbants.

Authors: T L Steck; J Yu
Journal: J Supramol Struct Date: 1973

8. A simple assay for the study of solubilized acetylcholine receptors.

Authors: J Schmidt; M A Raftery
Journal: Anal Biochem Date: 1973-04 Impact factor: 3.365

9. Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases.

Authors: L M Siegel; K J Monty
Journal: Biochim Biophys Acta Date: 1966-02-07

10. Changes in total and quantal release of acetylcholine in the mouse diaphragm during activation and inhibition of membrane ATPase.

Authors: E S Vizi; F Vyskocil
Journal: J Physiol Date: 1979-01 Impact factor: 5.182

22 in total

1. Phosphorylation of a peptide related to subunit c of the F0F1-ATPase/ATP synthase and relationship to permeability transition pore opening in mitochondria.

Authors: Tamara S Azarashvili; Jaana Tyynelä; Irina V Odinokova; Pavel A Grigorjev; Marc Baumann; Yuri V Evtodienko; Nils-Erik L Saris
Journal: J Bioenerg Biomembr Date: 2002-08 Impact factor: 2.945

10. Presynaptic K(+) channels, vesicular Ca(2+)/H (+) antiport--synaptotagmin, and acetylcholinesterase, three mechanisms cutting short the cholinergic signal at neuromuscular and nerve-electroplaque junctions.

Authors: Yves Dunant; J Miguel Cordeiro
Journal: J Mol Neurosci Date: 2014-01-04 Impact factor: 3.444

Purification of a presynaptic membrane protein that mediates a calcium-dependent translocation of acetylcholine.

1. Transmitter leakage from motor nerve endings.

2. Isolation of a chloroplast N,N'-dicyclohexylcarbodiimide-binding proteolipid, active in proton translocation.

3. A new and rapid colorimetric determination of acetylcholinesterase activity.

4. [Changes in acetylcholine level and electrophysiological response during continuous stimulation of the electric organ of Torpedo marmorata (author's transl)].

Review 5. The present status of the vesicular hypothesis.

6. Proteolipids from membrane systems.

7. Selective solubilization of proteins from red blood cell membranes by protein perturbants.

8. A simple assay for the study of solubilized acetylcholine receptors.

9. Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases.

10. Changes in total and quantal release of acetylcholine in the mouse diaphragm during activation and inhibition of membrane ATPase.

1. Phosphorylation of a peptide related to subunit c of the F0F1-ATPase/ATP synthase and relationship to permeability transition pore opening in mitochondria.

2. Acetylcholine release in rapid synapses: two fast partners--mediatophore and vesicular Ca2+/H+ antiport.

Review 3. Vacuolar H(+)-ATPase-an enzyme for all seasons.

Review 4. Ultra-fast versus sustained cholinergic transmission: a variety of different mechanisms.

5. Quantal acetylcholine release induced by mediatophore transfection.

Review 6. Acetylcholine release and the cholinergic genomic locus.

Review 7. In vitro reconstitution of neurotransmitter release.

Review 8. The membrane domain of vacuolar H(+)ATPase: a crucial player in neurotransmitter exocytotic release.

9. Activation and desensitisation of acetylcholine release by zinc at Torpedo nerve terminals.

10. Presynaptic K(+) channels, vesicular Ca(2+)/H (+) antiport--synaptotagmin, and acetylcholinesterase, three mechanisms cutting short the cholinergic signal at neuromuscular and nerve-electroplaque junctions.