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Literature DB >> 8643501

In vivo suppression of the renal Na+/Pi cotransporter by antisense oligonucleotides.

R Oberbauer¹, G F Schreiner, J Biber, H Murer, T W Meyer.

Abstract

A 20-mer phosphorothioate oligonucleotide (AS1) was designed to hybridize to the message for the rat kidney sodium phosphate cotransporter NaPi-2 close to the translation initiation site. Single intravenous doses of this oligonucleotide were given to rats maintained on a low phosphorus diet to increase NaPi-2 expression. At 3 days after oligonucleotide infusion, rats receiving 2.5 micromol of AS1 exhibited a reduction in renal NaPi-2 to cyclophilin mRNA ratio by 40% +/- 17%, and rats receiving 7.5 micromol of AS1 exhibited a reduction in NaPi-2 to cyclophilin mRNA ratio by 46% +/- 21%. Reversed-sequence AS1 was without effect. The higher dose of 7.5 micromol of AS1 also reduced the rate of phosphate uptake into renal brush border membrane vesicles and the expression of NaPi-2 protein detected by Western blotting in these vesicles. Reversed sequence AS1 was again without effect on these parameters. These results suggest that systemically infused oligonucleotides can exert antisense effects in the renal proximal tubule.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1996 PMID： 8643501 PMCID： PMC39377 DOI： 10.1073/pnas.93.10.4903

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

28 in total

Review 1. Structural and functional features of protein handling in the kidney proximal tubule.

Authors: E I Christensen; S Nielsen
Journal: Semin Nephrol Date: 1991-07 Impact factor: 5.299

2. Intracellular distribution of microinjected antisense oligonucleotides.

Authors: J P Leonetti; N Mechti; G Degols; C Gagnor; B Lebleu
Journal: Proc Natl Acad Sci U S A Date: 1991-04-01 Impact factor: 11.205

3. p1B15: a cDNA clone of the rat mRNA encoding cyclophilin.

Authors: P E Danielson; S Forss-Petter; M A Brow; L Calavetta; J Douglass; R J Milner; J G Sutcliffe
Journal: DNA Date: 1988-05

4. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors: H Towbin; T Staehelin; J Gordon
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

5. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

6. Mechanism of oligonucleotide uptake by cells: involvement of specific receptors?

Authors: L A Yakubov; E A Deeva; V F Zarytova; E M Ivanova; A S Ryte; L V Yurchenko; V V Vlassov
Journal: Proc Natl Acad Sci U S A Date: 1989-09 Impact factor: 11.205

7. A high yield preparation for rat kidney brush border membranes. Different behaviour of lysosomal markers.

Authors: J Biber; B Stieger; W Haase; H Murer
Journal: Biochim Biophys Acta Date: 1981-10-02

8. Pharmacokinetics, biodistribution, and stability of oligodeoxynucleotide phosphorothioates in mice.

Authors: S Agrawal; J Temsamani; J Y Tang
Journal: Proc Natl Acad Sci U S A Date: 1991-09-01 Impact factor: 11.205

9. Sodium-dependent phosphate transport by apical membrane vesicles from a cultured renal epithelial cell line (LLC-PK1).

Authors: C D Brown; M Bodmer; J Biber; H Murer
Journal: Biochim Biophys Acta Date: 1984-01-25

10. Chronic hepatitis, hepatocyte fragility, and increased soluble phosphoglycokeratins in transgenic mice expressing a keratin 18 conserved arginine mutant.

Authors: N O Ku; S Michie; R G Oshima; M B Omary
Journal: J Cell Biol Date: 1995-12 Impact factor: 10.539

8 in total

In vivo suppression of the renal Na+/Pi cotransporter by antisense oligonucleotides.

Review 1. Structural and functional features of protein handling in the kidney proximal tubule.

2. Intracellular distribution of microinjected antisense oligonucleotides.

3. p1B15: a cDNA clone of the rat mRNA encoding cyclophilin.

4. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

5. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

6. Mechanism of oligonucleotide uptake by cells: involvement of specific receptors?

7. A high yield preparation for rat kidney brush border membranes. Different behaviour of lysosomal markers.

8. Pharmacokinetics, biodistribution, and stability of oligodeoxynucleotide phosphorothioates in mice.

9. Sodium-dependent phosphate transport by apical membrane vesicles from a cultured renal epithelial cell line (LLC-PK1).

10. Chronic hepatitis, hepatocyte fragility, and increased soluble phosphoglycokeratins in transgenic mice expressing a keratin 18 conserved arginine mutant.

Review 1. The renal type II Na+/phosphate cotransporter.

2. Amino acids involved in sodium interaction of murine type II Na(+)-P(i) cotransporters expressed in Xenopus oocytes.

Review 3. Hypophosphatemic rickets: lessons from disrupted FGF23 control of phosphorus homeostasis.

4. Lack of the growth factor midkine enhances survival against cisplatin-induced renal damage.

Review 5. Fomivirsen: clinical pharmacology and potential drug interactions.

Review 6. Renal Ciliopathies: Sorting Out Therapeutic Approaches for Nephronophthisis.

Review 7. Renal gene transfer: nonviral approaches.

8. In Vitro Selection of Cell-Internalizing DNA Aptamers in a Model System of Inflammatory Kidney Disease.