Literature DB >> 8641761

Molecular characterization of a ribonucleotide reductase (nrdF) gene fragment of Mycoplasma hyopneumoniae and assessment of the recombinant product as an experimental vaccine for enzootic pneumonia.

P K Fagan1, S P Djordjevic, G J Eamens, J Chin, M J Walker.   

Abstract

A Mycoplasma hyopneumoniae clone bank was screened with hyperimmune pig serum. One clone exhibited sequence homology to the prokaryotic R2 subunit of ribonucleotide reductase and was expressed as an 11-kDa protein fused to beta-galactosidase. The vaccine potential of the fusion protein was assessed in pig trials. Following experimental challenge with a virulent isolate of M. hyopneumoniae, gross lung pathology (mean Goodwin lung score) of vaccinated animals, irrespective of adjuvant treatment, was significantly reduced compared with that of control unvaccinated pigs (P < 0.05).

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Year:  1996        PMID： 8641761      PMCID： PMC173882          DOI： 10.1128/iai.64.3.1060-1064.1996

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  32 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

Review 2.  Reduction of ribonucleotides.

Authors:  L Thelander; P Reichard
Journal:  Annu Rev Biochem       Date:  1979       Impact factor: 23.643

3.  Swine enzootic pneumonia: incidence and effect on rate of body weight gain.

Authors:  R G Huhn
Journal:  Am J Vet Res       Date:  1970-06       Impact factor: 1.156

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Immunity in experimentally induced enzootic pneumonia in pigs.

Authors:  R F Goodwin; R G Hodgson; P Whittlestone; R L Woodhams
Journal:  J Hyg (Lond)       Date:  1969-06

6.  Aerosol vaccination of pigs against Mycoplasma hyopneumoniae infection.

Authors:  D Murphy; W G Van Alstine; L K Clark; S Albregts; K Knox
Journal:  Am J Vet Res       Date:  1993-11       Impact factor: 1.156

7.  Cloning and sequencing of the genes from Salmonella typhimurium encoding a new bacterial ribonucleotide reductase.

Authors:  A Jordan; I Gibert; J Barbé
Journal:  J Bacteriol       Date:  1994-06       Impact factor: 3.490

8.  A survey of the Mycoplasma genitalium genome by using random sequencing.

Authors:  S N Peterson; P C Hu; K F Bott; C A Hutchison
Journal:  J Bacteriol       Date:  1993-12       Impact factor: 3.490

9.  Mycoplasma hyopneumoniae infection in pigs: duration of the disease and resistance to reinfection.

Authors:  M Kobisch; B Blanchard; M F Le Potier
Journal:  Vet Res       Date:  1993       Impact factor: 3.683

10.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205
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  5 in total

1.  Sequence TTKF ↓ QE defines the site of proteolytic cleavage in Mhp683 protein, a novel glycosaminoglycan and cilium adhesin of Mycoplasma hyopneumoniae.

Authors:  Daniel R Bogema; Nichollas E Scott; Matthew P Padula; Jessica L Tacchi; Benjamin B A Raymond; Cheryl Jenkins; Stuart J Cordwell; F Chris Minion; Mark J Walker; Steven P Djordjevic
Journal:  J Biol Chem       Date:  2011-10-03       Impact factor: 5.157

2.  A processed multidomain mycoplasma hyopneumoniae adhesin binds fibronectin, plasminogen, and swine respiratory cilia.

Authors:  Lisa M Seymour; Ania T Deutscher; Cheryl Jenkins; Tracey A Kuit; Linda Falconer; F Chris Minion; Ben Crossett; Matthew Padula; Nicholas E Dixon; Steven P Djordjevic; Mark J Walker
Journal:  J Biol Chem       Date:  2010-09-02       Impact factor: 5.157

3.  Oral immunization of mice with attenuated Salmonella typhimurium aroA expressing a recombinant Mycoplasma hyopneumoniae antigen (NrdF).

Authors:  P K Fagan; S P Djordjevic; J Chin; G J Eamens; M J Walker
Journal:  Infect Immun       Date:  1997-06       Impact factor: 3.441

Review 4.  Molecular biology and pathogenicity of mycoplasmas.

Authors:  S Razin; D Yogev; Y Naot
Journal:  Microbiol Mol Biol Rev       Date:  1998-12       Impact factor: 11.056

5.  Development of non-antibiotic-resistant, chromosomally based, constitutive and inducible expression systems for aroA-attenuated Salmonella enterica Serovar Typhimurium.

Authors:  Jake N Matic; Tamsin D Terry; David Van Bockel; Tracy Maddocks; David Tinworth; Michael P Jennings; Steven P Djordjevic; Mark J Walker
Journal:  Infect Immun       Date:  2009-02-17       Impact factor: 3.441
  5 in total

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