OBJECTIVE: To compare the detection rates of chromosomal flagellin gene from Borrelia burgdorferi in synovial tissue (ST) and synovial fluid (SF) using polymerase chain reaction (PCR) techniques. METHODS: B burgdorferi DNA was sought in SF and ST from 12 consecutive patients with Lyme arthritis and from 29 patients with noninfectious diseases (controls). RESULTS: No DNA amplification was observed in samples obtained from the 29 control patients, whereas B burgdorferi DNA was detected in all ST and/or SF samples from the 12 patients with Lyme arthritis. Results from 1 ST sample were not interpretable because of PCR inhibitors. Among the 11 remaining patients, 10 had positive ST samples, whereas only 4 had positive SF samples (P < 0.05). CONCLUSION: These data suggest that detection of chromosomal B burgdorferi DNA may be more efficient in ST than SF.
OBJECTIVE: To compare the detection rates of chromosomal flagellin gene from Borrelia burgdorferi in synovial tissue (ST) and synovial fluid (SF) using polymerase chain reaction (PCR) techniques. METHODS: B burgdorferi DNA was sought in SF and ST from 12 consecutive patients with Lyme arthritis and from 29 patients with noninfectious diseases (controls). RESULTS: No DNA amplification was observed in samples obtained from the 29 control patients, whereas B burgdorferi DNA was detected in all ST and/or SF samples from the 12 patients with Lyme arthritis. Results from 1 ST sample were not interpretable because of PCR inhibitors. Among the 11 remaining patients, 10 had positive ST samples, whereas only 4 had positive SF samples (P < 0.05). CONCLUSION: These data suggest that detection of chromosomal B burgdorferi DNA may be more efficient in ST than SF.
Authors: B Jaulhac; R Heller; F X Limbach; Y Hansmann; D Lipsker; H Monteil; J Sibilia; Y Piémont Journal: J Clin Microbiol Date: 2000-05 Impact factor: 5.948
Authors: J G Kuipers; J Sibilia; S Bas; H Gaston; K Granfors; T L Vischer; N Hajjaj-Hassouni; A Ladjouze-Rezig; S Sellami; J Wollenhaupt; H Zeidler; H R Schumacher; M Dougados Journal: Clin Rheumatol Date: 2008-08-08 Impact factor: 2.980