Literature DB >> 10325373

One-step reverse transcriptase PCR method for detection of Borrelia burgdorferi mRNA in mouse Lyme arthritis tissue samples.

F X Limbach1, B Jaulhac, Y Piémont, J L Kuntz, H Monteil, J Sibilia.   

Abstract

A one-step reverse transcriptase PCR (RT-PCR) method for detection of Borrelia burgdorferi mRNA in infected C3H mice is described. This simple procedure, less prone to nucleic acid cross-contamination than the standard method, was found to be 10-fold more sensitive than a classical two-step RT-PCR assay. By using one-step RT-PCR, flagellin mRNAs were detected in synovial and heart tissues from all seven infected mice tested.

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Year:  1999        PMID： 10325373      PMCID： PMC85022     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  15 in total

1.  Detection of Borrelia burgdorferi in cerebrospinal fluid of patients with Lyme borreliosis.

Authors:  B Jaulhac; P Nicolini; Y Piemont; H Monteil
Journal:  N Engl J Med       Date:  1991-05-16       Impact factor: 91.245

2.  Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase.

Authors:  T W Myers; D H Gelfand
Journal:  Biochemistry       Date:  1991-08-06       Impact factor: 3.162

3.  Detection of Borrelia burgdorferi by DNA amplification in synovial tissue samples from patients with Lyme arthritis.

Authors:  B Jaulhac; I Chary-Valckenaere; J Sibilia; R M Javier; Y Piémont; J L Kuntz; H Monteil; J Pourel
Journal:  Arthritis Rheum       Date:  1996-05

4.  Ultrastructural demonstration of spirochetal antigens in synovial fluid and synovial membrane in chronic Lyme disease: possible factors contributing to persistence of organisms.

Authors:  R Nanagara; P H Duray; H R Schumacher
Journal:  Hum Pathol       Date:  1996-10       Impact factor: 3.466

5.  Temporal pattern of Borrelia burgdorferi p21 expression in ticks and the mammalian host.

Authors:  S Das; S W Barthold; S S Giles; R R Montgomery; S R Telford; E Fikrig
Journal:  J Clin Invest       Date:  1997-03-01       Impact factor: 14.808

6.  Fate of Borrelia burgdorferi DNA in tissues of infected mice after antibiotic treatment.

Authors:  S E Malawista; S W Barthold; D H Persing
Journal:  J Infect Dis       Date:  1994-11       Impact factor: 5.226

7.  Borrelia burgdorferi genes selectively expressed in the infected host.

Authors:  K Suk; S Das; W Sun; B Jwang; S W Barthold; R A Flavell; E Fikrig
Journal:  Proc Natl Acad Sci U S A       Date:  1995-05-09       Impact factor: 11.205

8.  Heritable susceptibility to severe Borrelia burgdorferi-induced arthritis is dominant and is associated with persistence of large numbers of spirochetes in tissues.

Authors:  L Yang; J H Weis; E Eichwald; C P Kolbert; D H Persing; J J Weis
Journal:  Infect Immun       Date:  1994-02       Impact factor: 3.441

9.  Antigenic stability of Borrelia burgdorferi during chronic infections of immunocompetent mice.

Authors:  S W Barthold
Journal:  Infect Immun       Date:  1993-12       Impact factor: 3.441

10.  Direct demonstration of antigenic substitution of Borrelia burgdorferi ex vivo: exploration of the paradox of the early immune response to outer surface proteins A and C in Lyme disease.

Authors:  R R Montgomery; S E Malawista; K J Feen; L K Bockenstedt
Journal:  J Exp Med       Date:  1996-01-01       Impact factor: 14.307
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  1 in total

1.  A semi-quantitative RT-PCR method to readily compare expression levels within Botrytis cinerea multigenic families in vitro and in planta.

Authors:  Mathias Choquer; Martine Boccara; Anne Vidal-Cros
Journal:  Curr Genet       Date:  2003-05-10       Impact factor: 3.886
  1 in total

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