Literature DB >> 8638106

A distinct G1 step required to specify the Chinese hamster DHFR replication origin.

J R Wu1, D M Gilbert.   

Abstract

Nuclei isolated from Chinese hamster ovary (CHO) cells at various times during the G1 phase of the cell cycle were stimulated to enter S phase by incubation in Xenopus egg cytosol. Replication of DNA initiated within the dihydrofolate reductase (DHFR) origin locus in nuclei isolated late in G1, but at random sites in nuclei isolated early in G1. A discrete transition point occurred 3 to 4 hours after metaphase. Neither replication licensing nor nuclear assembly was sufficient for origin recognition. Thus, a distinct cell cycle-regulated event in the nucleus restricts the initiation of replication to specific sites downstream of the DHFR gene.

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Year:  1996        PMID： 8638106     DOI： 10.1126/science.271.5253.1270

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  54 in total

1.  Activation of dormant origins of DNA replication in budding yeast.

Authors:  C Santocanale; K Sharma; J F Diffley
Journal:  Genes Dev       Date:  1999-09-15       Impact factor: 11.361

2.  Dynamics of association of origins of DNA replication with the nuclear matrix during the cell cycle.

Authors:  V Djeliova; G Russev; B Anachkova
Journal:  Nucleic Acids Res       Date:  2001-08-01       Impact factor: 16.971

3.  Cell cycle-dependent regulation of the association between origin recognition proteins and somatic cell chromatin.

Authors:  Wei-Hsin Sun; Thomas R Coleman; Melvin L DePamphilis
Journal:  EMBO J       Date:  2002-03-15       Impact factor: 11.598

4.  Stability, chromatin association and functional activity of mammalian pre-replication complex proteins during the cell cycle.

Authors:  Y Okuno; A J McNairn; N den Elzen; J Pines; D M Gilbert
Journal:  EMBO J       Date:  2001-08-01       Impact factor: 11.598

5.  Site-specific and temporally controlled initiation of DNA replication in a human cell-free system.

Authors:  Christian Keller; Olivier Hyrien; Rolf Knippers; Torsten Krude
Journal:  Nucleic Acids Res       Date:  2002-05-15       Impact factor: 16.971

6.  Selective instability of Orc1 protein accounts for the absence of functional origin recognition complexes during the M-G(1) transition in mammals.

Authors:  D A Natale; C J Li; W H Sun; M L DePamphilis
Journal:  EMBO J       Date:  2000-06-01       Impact factor: 11.598

7.  Sequence-independent DNA binding and replication initiation by the human origin recognition complex.

Authors:  Sanjay Vashee; Christin Cvetic; Wenyan Lu; Pamela Simancek; Thomas J Kelly; Johannes C Walter
Journal:  Genes Dev       Date:  2003-08-01       Impact factor: 11.361

Review 8.  In search of the holy replicator.

Authors:  David M Gilbert
Journal:  Nat Rev Mol Cell Biol       Date:  2004-10       Impact factor: 94.444

9.  Clusters, factories and domains: The complex structure of S-phase comes into focus.

Authors:  Peter J Gillespie; J Julian Blow
Journal:  Cell Cycle       Date:  2010-08-11       Impact factor: 4.534

Review 10.  DNA replication timing, genome stability and cancer: late and/or delayed DNA replication timing is associated with increased genomic instability.

Authors:  Nathan Donley; Mathew J Thayer
Journal:  Semin Cancer Biol       Date:  2013-01-14       Impact factor: 15.707
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