Literature DB >> 862624

Guinea-pig pancreatic ribonucleases. Isolation, properties, primary structure and glycosidation.

A Van den Berg, L Van den Hende-Timmer, J Hofsteenge, W Gaastra, J J Beintema.   

Abstract

Two ribonucleases were isolated from guinea-pig pancreas by extraction with 0.125 M sulfuric acid, precipitation with acetone and chromatography on carboxymethyl-cellulose. The amino acid sequences were determined from tryptic digests of the aminoethylated proteins. The tryptic peptides were positioned in the sequence by homology with other pancreatic ribonucleases. Both ribonucleases not only differ in the presence (ribonuclease B) or absence of carbohydrate (ribonuclease A), but also at 31 positions of the amino acid sequence. In guinea-pig ribonuclease B a leucine/proline heterogeneity was found at position 64. The carbohydrate in guinea-pig ribonuclease B is attached to asparagine residues at positions 21 and 34. The carbohydrate-free guinea-pig ribonuclease A possesses a recognition site for sugar attachment in the sequence Asn-Val-Ser (62-64).

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Year:  1977        PMID： 862624     DOI： 10.1111/j.1432-1033.1977.tb11507.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  6 in total

1.  The molecular evolution of pancreatic ribonuclease.

Authors:  J J Beintema; W Gaastra; J A Lenstra; G W Welling; W M Fitch
Journal:  J Mol Evol       Date:  1977-09-20       Impact factor: 2.395

2.  Role of proline peptide bond isomerization in unfolding and refolding of ribonuclease.

Authors:  F X Schmid; R Grafl; A Wrba; J J Beintema
Journal:  Proc Natl Acad Sci U S A       Date:  1986-02       Impact factor: 11.205

3.  Evolutionary changes in protein composition -- evidence for an optimal strategy.

Authors:  R Coutelle; G L Hofacker; R D Levine
Journal:  J Mol Evol       Date:  1979-06-08       Impact factor: 2.395

4.  Molecular structure of the immunity gene and immunity protein of the bacteriocinogenic plasmid Clo DF13.

Authors:  P J van den Elzen; W Gaastra; C E Spelt; F K de Graaf; E Veltkamp; H J Nijkamp
Journal:  Nucleic Acids Res       Date:  1980-10-10       Impact factor: 16.971

5.  Origin of the duplicated ribonuclease gene in guinea-pig: comparison of the amino acid sequences with those of two close relatives: capybara and cuis ribonuclease.

Authors:  J J Beintema; B Neuteboom
Journal:  J Mol Evol       Date:  1983       Impact factor: 2.395

6.  The expansion and functional diversification of the mammalian ribonuclease a superfamily epitomizes the efficiency of multigene families at generating biological novelty.

Authors:  Stephen M Goo; Soochin Cho
Journal:  Genome Biol Evol       Date:  2013       Impact factor: 3.416
  6 in total

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