Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 A cyanobacterial narB gene encodes a ferredoxin-dependent nitrate reductase.

Literature DB >> 8624415

A cyanobacterial narB gene encodes a ferredoxin-dependent nitrate reductase.

Abstract

The narB gene from the cyanobacterium Synechococcus sp. PCC 7942 was cloned downstream from the LacI-regulated promoter Ptrc in the Escherichia coli vector pTrc99A, rendering plasmid pCSLM1. Addition of isopropyl-beta-D-thiogalactoside to E. coli (pCSLM1) resulted in the parallel expression of a 76 kDa polypeptide and a nitrate reductase activity with properties identical to those known for nitrate reductase isolated from Synechococcus cells. As is the case for nitrate reductase from Synechococcus cells, either reduced methyl viologen or reduced ferredoxin could be used as an electron donor for the reduction of nitrate catalyzed by E. coli (pCSLM1) extracts. This data shows that narB is a cyanobacterial structural gene for nitrate reductase.

Entities: Chemical Species

Mesh：

Substances：

Year: 1996 PMID： 8624415 DOI： 10.1007/bf00019017

Source DB: PubMed Journal: Plant Mol Biol ISSN： 0167-4412 Impact factor: 4.076

15 in total

Review 1. The pterin molybdenum cofactors.

Authors: K V Rajagopalan; J L Johnson
Journal: J Biol Chem Date: 1992-05-25 Impact factor: 5.157

2. Biphasic kinetic behavior of nitrate reductase from heterocystous, nitrogen-fixing cyanobacteria.

Authors: J Martin-Nieto; E Flores; A Herrero
Journal: Plant Physiol Date: 1992-09 Impact factor: 8.340

Review 3. The molecular genetics of nitrate assimilation in fungi and plants.

Authors: N M Crawford; H N Arst
Journal: Annu Rev Genet Date: 1993 Impact factor: 16.830

4. Cloning of a third nitrate reductase gene from the cyanobacterium Anacystis nidulans R2 using a shuttle cosmid library.

Authors: C J Kuhlemeier; V J Teeuwsen; M J Janssen; G A van Arkel
Journal: Gene Date: 1984-11 Impact factor: 3.688

5. Nitrite reductase gene from Synechococcus sp. PCC 7942: homology between cyanobacterial and higher-plant nitrite reductases.

Authors: I Luque; E Flores; A Herrero
Journal: Plant Mol Biol Date: 1993-03 Impact factor: 4.076

6. Structures of genes nasA and nasB, encoding assimilatory nitrate and nitrite reductases in Klebsiella pneumoniae M5al.

Authors: J T Lin; B S Goldman; V Stewart
Journal: J Bacteriol Date: 1993-04 Impact factor: 3.490

7. Purification and characterization of the assimilatory nitrate reductase of Azotobacter vinelandii.

Authors: R Gangeswaran; D J Lowe; R R Eady
Journal: Biochem J Date: 1993-01-15 Impact factor: 3.857

8. Cloning of nitrate reductase genes from the cyanobacterium Anacystis nidulans.

Authors: C J Kuhlemeier; T Logtenberg; W Stoorvogel; H A van Heugten; W E Borrias; G A van Arkel
Journal: J Bacteriol Date: 1984-07 Impact factor: 3.490

9. Regulation of nitrate reductase levels in the cyanobacteria Anacystis nidulans, Anabaena sp. strain 7119, and Nostoc sp. strain 6719.

Authors: A Herrero; E Flores; M G Guerrero
Journal: J Bacteriol Date: 1981-01 Impact factor: 3.490

10. Molecular mechanism for the operation of nitrogen control in cyanobacteria.

Authors: I Luque; E Flores; A Herrero
Journal: EMBO J Date: 1994-06-15 Impact factor: 11.598

20 in total

Review 1. Nitrogen control in cyanobacteria.

Authors: A Herrero; A M Muro-Pastor; E Flores
Journal: J Bacteriol Date: 2001-01 Impact factor: 3.490

Review 2. Prokaryotic nitrate reduction: molecular properties and functional distinction among bacterial nitrate reductases.

Authors: C Moreno-Vivián; P Cabello; M Martínez-Luque; R Blasco; F Castillo
Journal: J Bacteriol Date: 1999-11 Impact factor: 3.490

3. Open reading frame all0601 from Anabaena sp. strain PCC 7120 represents a novel gene, cnaT, required for expression of the nitrate assimilation nir operon.

Authors: José E Frías; Antonia Herrero; Enrique Flores
Journal: J Bacteriol Date: 2003-09 Impact factor: 3.490

4. A novel gene (narM) required for expression of nitrate reductase activity in the cyanobacterium Synechococcus elongatus strain PCC7942.

Authors: Shin-ichi Maeda; Tatsuo Omata
Journal: J Bacteriol Date: 2004-04 Impact factor: 3.490

5. Application of the Synechococcus nirA promoter to establish an inducible expression system for engineering the Synechocystis tocopherol pathway.

Authors: Qungang Qi; Ming Hao; Wing-On Ng; Steven C Slater; Susan R Baszis; James D Weiss; Henry E Valentin
Journal: Appl Environ Microbiol Date: 2005-10 Impact factor: 4.792

6. NO3-/NO2- assimilation in halophilic archaea: physiological analysis, nasA and nasD expressions.

Authors: Rosa María Martínez-Espinosa; Belén Lledó; Frutos C Marhuenda-Egea; Susana Díaz; María José Bonete
Journal: Extremophiles Date: 2009-07-11 Impact factor: 2.395

7. Presence of a [3Fe-4S] cluster in a PsaC variant as a functional component of the photosystem I electron transfer chain in Synechococcus sp. PCC 7002.

Authors: Adam A Pérez; Bryan H Ferlez; Amanda M Applegate; Karim Walters; Zhihui He; Gaozhong Shen; John H Golbeck; Donald A Bryant
Journal: Photosynth Res Date: 2017-09-15 Impact factor: 3.573

Review 8. Photosynthetic nitrate assimilation in cyanobacteria.

Authors: Enrique Flores; José E Frías; Luis M Rubio; Antonia Herrero
Journal: Photosynth Res Date: 2005 Impact factor: 3.573

9. Induction of the Nitrate Assimilation nirA Operon and Protein-Protein Interactions in the Maturation of Nitrate and Nitrite Reductases in the Cyanobacterium Anabaena sp. Strain PCC 7120.

Authors: José E Frías; Enrique Flores
Journal: J Bacteriol Date: 2015-05-11 Impact factor: 3.490

10. Monomeric NarB is a dual-affinity nitrate reductase, and its activity is regulated differently from that of nitrate uptake in the unicellular diazotrophic cyanobacterium Synechococcus sp. strain RF-1.

Authors: Tung-Hei Wang; Hongyong Fu; Yuh-Jang Shieh
Journal: J Bacteriol Date: 2003-10 Impact factor: 3.490