Literature DB >> 6098517

Cloning of a third nitrate reductase gene from the cyanobacterium Anacystis nidulans R2 using a shuttle cosmid library.

C J Kuhlemeier, V J Teeuwsen, M J Janssen, G A van Arkel.   

Abstract

A strategy for gene cloning in the cyanobacterium Anacystis nidulans R2 was developed which made use of a gene library constructed in a shuttle cosmid vector. The method involved phenotypic complementation of mutants with pooled cosmid DNA. The development of the procedure and its application to the cloning of a third gene involved in nitrate reduction are described.

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Year:  1984        PMID： 6098517     DOI： 10.1016/0378-1119(84)90200-2

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  11 in total

1.  A novel gene (narM) required for expression of nitrate reductase activity in the cyanobacterium Synechococcus elongatus strain PCC7942.

Authors:  Shin-ichi Maeda; Tatsuo Omata
Journal:  J Bacteriol       Date:  2004-04       Impact factor: 3.490

2.  Clustering of genes involved in nitrate assimilation in the cyanobacterium Synechococcus.

Authors:  I Luque; A Herrero; E Flores; F Madueño
Journal:  Mol Gen Genet       Date:  1992-03

3.  Identification and cloning of a regulatory gene for nitrogen assimilation in the cyanobacterium Synechococcus sp. strain PCC 7942.

Authors:  M A Vega-Palas; F Madueño; A Herrero; E Flores
Journal:  J Bacteriol       Date:  1990-02       Impact factor: 3.490

4.  Physical and genetic maps of the genome of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120.

Authors:  I Bancroft; C P Wolk; E V Oren
Journal:  J Bacteriol       Date:  1989-11       Impact factor: 3.490

5.  A cyanobacterial narB gene encodes a ferredoxin-dependent nitrate reductase.

Authors:  L M Rubio; A Herrero; E Flores
Journal:  Plant Mol Biol       Date:  1996-02       Impact factor: 4.076

6.  Identification and characterization of two nitrogen-regulated genes of the cyanobacterium Synechococcus sp. strain PCC7942 required for maximum efficiency of nitrogen assimilation.

Authors:  I Suzuki; N Horie; T Sugiyama; T Omata
Journal:  J Bacteriol       Date:  1995-01       Impact factor: 3.490

7.  The narA locus of Synechococcus sp. strain PCC 7942 consists of a cluster of molybdopterin biosynthesis genes.

Authors:  L M Rubio; E Flores; A Herrero
Journal:  J Bacteriol       Date:  1998-03       Impact factor: 3.490

8.  Controlled gene expression utilising lambda phage regulatory signals in a cyanobacterium host.

Authors:  D Friedberg; J Seijffers
Journal:  Mol Gen Genet       Date:  1986-06

9.  Nitrate assimilation gene cluster from the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120.

Authors:  J E Frías; E Flores; A Herrero
Journal:  J Bacteriol       Date:  1997-01       Impact factor: 3.490

10.  A nitrate reductase gene of the cyanobacterium Synechococcus PCC6301 inferred by heterologous hybridization, cloning and targeted mutagenesis.

Authors:  D A Lightfoot; A J Baron; J M Cock; J C Wootton
Journal:  Genetica       Date:  1992       Impact factor: 1.082
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