Literature DB >> 8614618

The use of non-uniform deuterium labelling ['NMR-window'] to study the NMR structure of a 21mer RNA hairpin.

A Földesi1, S I Yamakage, F P Nilsson, T V Maltseva, J Chattopadhyaya.   

Abstract

The first synthesis of a non-uniformly deuterium labelled 21mer RNA is reported using our 'NMR-window' concept, showing its unique application in the unambiguous NMR assignment of the non-exchangeable aromatic and sugar protons.

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Year:  1996        PMID： 8614618      PMCID： PMC145781          DOI： 10.1093/nar/24.7.1187

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  18 in total

1.  NMR analysis of helix I from the 5S RNA of Escherichia coli.

Authors:  S A White; M Nilges; A Huang; A T Brünger; P B Moore
Journal:  Biochemistry       Date:  1992-02-18       Impact factor: 3.162

Review 2.  Novel techniques in nuclear magnetic resonance for nucleic acids.

Authors:  F Aboul-ela; G Varani
Journal:  Curr Opin Biotechnol       Date:  1995-02       Impact factor: 9.740

3.  Cysteine-catalyzed hydrogen isotope exchange at the 5 position of uridylic acid.

Authors:  Y Wataya; H Hayatsu; Y Kawazoe
Journal:  J Am Chem Soc       Date:  1972-12-13       Impact factor: 15.419

4.  Nucleosides. LXXIX. Facile base-catalyzed hydrogen isotope labeling at position 6 of pyrimidine nucleosides.

Authors:  J A Rabi; J J Fox
Journal:  J Am Chem Soc       Date:  1973-03-07       Impact factor: 15.419

5.  1-H NMR studies on deuterium-hydrogen exchange at C-5 in uridines.

Authors:  S R Heller
Journal:  Biochem Biophys Res Commun       Date:  1968-09-30       Impact factor: 3.575

6.  Selective deuteration of RNA for NMR signal assignment.

Authors:  I Kim; S Watanabe; Y Muto; K Hosono; K Takai; H Takaku; G Kawai; K Watanabe; S Yokoyama
Journal:  Nucleic Acids Symp Ser       Date:  1995

7.  Pyridyl groups for protection of the imide functions of uridine and guanosine. Exploration of their displacement reactions for site-specific modifications of uracil and guanine bases.

Authors:  X X Zhou; C J Welch; J Chattopadhyaya
Journal:  Acta Chem Scand B       Date:  1986-11

8.  Systematic application of high-resolution, phase-sensitive two-dimensional 1H-NMR techniques for the identification of the amino-acid-proton spin systems in proteins. Rabbit metallothionein-2.

Authors:  D Neuhaus; G Wagner; M Vasák; J H Kägi; K Wüthrich
Journal:  Eur J Biochem       Date:  1985-09-02

9.  Removal of t-butyldimethylsilyl protection in RNA-synthesis. Triethylamine trihydrofluoride (TEA, 3HF) is a more reliable alternative to tetrabutylammonium fluoride (TBAF).

Authors:  E Westman; R Strömberg
Journal:  Nucleic Acids Res       Date:  1994-06-25       Impact factor: 16.971

10.  Conformation of an RNA pseudoknot.

Authors:  J D Puglisi; J R Wyatt; I Tinoco
Journal:  J Mol Biol       Date:  1990-07-20       Impact factor: 5.469
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  7 in total

1.  Preparation of partially 2H/13C-labelled RNA for NMR studies. Stereo-specific deuteration of the H5" in nucleotides.

Authors:  Jenny Cromsigt; Jürgen Schleucher; Tomas Gustafsson; Jan Kihlberg; Sybren Wijmenga
Journal:  Nucleic Acids Res       Date:  2002-04-01       Impact factor: 16.971

2.  Interactions of a didomain fragment of the Drosophila sex-lethal protein with single-stranded uridine-rich oligoribonucleotides derived from the transformer and Sex-lethal messenger RNA precursors: NMR with residue-selective [5-2H]uridine substitutions.

Authors:  I Kim; Y Muto; S Watanabe; A Kitamura; Y Futamura; S Yokoyama; K Hosono; G Kawai; H Takaku; N Dohmae; K Takio; H Saskamoto; Y Shimura
Journal:  J Biomol NMR       Date:  2000-06       Impact factor: 2.835

3.  Specific labeling approaches to guanine and adenine imino and amino proton assignments in the AMP-RNA aptamer complex.

Authors:  F Jiang; D J Patel; X Zhang; H Zhao; R A Jones
Journal:  J Biomol NMR       Date:  1997-01       Impact factor: 2.835

4.  The NMR structure of 31mer RNA domain of Escherichia coli RNase P RNA using its non-uniformly deuterium labelled counterpart [the 'NMR-window' concept].

Authors:  C Glemarec; J Kufel; A Földesi; T Maltseva; A Sandström; L A Kirsebom; J Chattopadhyaya
Journal:  Nucleic Acids Res       Date:  1996-06-01       Impact factor: 16.971

5.  An efficient and economic site-specific deuteration strategy for NMR studies of homologous oligonucleotide repeat sequences.

Authors:  X Huang; P Yu; E LeProust; X Gao
Journal:  Nucleic Acids Res       Date:  1997-12-01       Impact factor: 16.971

6.  Synthesis and incorporation of 13C-labeled DNA building blocks to probe structural dynamics of DNA by NMR.

Authors:  Felix Nußbaumer; Michael Andreas Juen; Catherina Gasser; Johannes Kremser; Thomas Müller; Martin Tollinger; Christoph Kreutz
Journal:  Nucleic Acids Res       Date:  2017-09-06       Impact factor: 16.971

7.  Preparation of selective and segmentally labeled single-stranded DNA for NMR by self-primed PCR and asymmetrical endonuclease double digestion.

Authors:  Frank H T Nelissen; Frederic C Girard; Marco Tessari; Hans A Heus; Sybren S Wijmenga
Journal:  Nucleic Acids Res       Date:  2009-06-24       Impact factor: 16.971
  7 in total

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