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Literature DB >> 8613399

Passive immunization with antiserum to a nontoxic alpha-toxin mutant from Staphylococcus aureus is protective in a murine model.

Abstract

A nonhemolytic, nonlethal variant of Staphylococcus aureus alpha-toxin constructed via oligonucleotide-directed mutagenesis and containing a single amino acid substitution (H-35 to L) was used to immunize a rabbit. The resulting antiserum was cross-reactive with wild-type alpha-toxin and neutralized its hemolytic activity in vitro. Passive immunization of mice with rabbit antiserum conferred protection against lethal challenge with wild-type alpha-toxin and against acute lethal challenge with a high-alpha-toxin -producing S. aureus strain. H35L alpha-toxin may be useful as a protective immunogen in S. aureus vaccine studies.

Entities: Chemical Mutation Species

Mesh：

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Year: 1996 PMID： 8613399 PMCID： PMC174000 DOI： 10.1128/iai.64.5.1839-1841.1996

Source DB: PubMed Journal: Infect Immun ISSN： 0019-9567 Impact factor: 3.441

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