Literature DB >> 1270140

Purification of staphylococcal alpha-toxin by adsorption chromatography on glass.

P Cassidy, S Harshman.   

Abstract

Staphylococcal alpha-toxin was purified from Staphylococcus aureus growth medium using adsorption chromatography on controlled pore glass beads. Elution of alpha-toxin from the unmodified glass surface of the beads with various anions generally followed the chaotropic series. Alpha-toxin, purified by glass bead chromatography, is composed of a single electrophoretic form, containing less than 2% of other forms.

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Year:  1976        PMID： 1270140      PMCID： PMC420702          DOI： 10.1128/iai.13.3.982-986.1976

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  11 in total

1.  Isolation and composition of staphylococcal alpha toxin.

Authors:  A W BERNHEIMER; L L SCHWARTZ
Journal:  J Gen Microbiol       Date:  1963-03

2.  Purification and some properties of staphylococcal alpha-toxin.

Authors:  M Watanabe; I Kato
Journal:  Jpn J Exp Med       Date:  1974-04

3.  Destabilization of membranes with chaotropic ions.

Authors:  Y Hatefi; W G Hanstein
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

4.  Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.

Authors:  G Fairbanks; T L Steck; D F Wallach
Journal:  Biochemistry       Date:  1971-06-22       Impact factor: 3.162

5.  Application of permeation chromatography on controlled-pore glass in the purification of plant viruses.

Authors:  K Marcinka
Journal:  Acta Virol       Date:  1972-06       Impact factor: 1.162

6.  Chromatography of biological materials on polyethylene glycol-treated controlled-pore glass.

Authors:  G L Hawk; J A Cameron; L B Dufault
Journal:  Prep Biochem       Date:  1972

7.  Purification and properties of two forms of staphylococcal toxin.

Authors:  H R Six; S Harshman
Journal:  Biochemistry       Date:  1973-07-03       Impact factor: 3.162

8.  Purification of D-beta-hydroxybutyrate apodehydrogenase, a lecithin-requiring enzyme.

Authors:  H O Bock; S Fleischer
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

9.  Physical and chemical studies on staphylococcal -toxins A and B .

Authors:  H R Six; S Harshman
Journal:  Biochemistry       Date:  1973-07-03       Impact factor: 3.162

10.  Some biological properties of purified staphylococcal haemolysins.

Authors:  T Wadström; R Möllby
Journal:  Toxicon       Date:  1972-08       Impact factor: 3.033
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  18 in total

Review 1.  Nonenteric toxins of Staphylococcus aureus.

Authors:  M Rogolsky
Journal:  Microbiol Rev       Date:  1979-09

2.  Phospholipase D activity of Vibrio damsela cytolysin and its interaction with sheep erythrocytes.

Authors:  A S Kreger; A W Bernheimer; L A Etkin; L W Daniel
Journal:  Infect Immun       Date:  1987-12       Impact factor: 3.441

3.  Loop Diuretics Diminish Hemolysis Induced by α-Hemolysin from Escherichia coli.

Authors:  Carl Martin Söderström; Steen K Fagerberg; Mette B Brogaard; Jens Leipziger; Marianne Skals; Helle A Praetorius
Journal:  J Membr Biol       Date:  2017-05-09       Impact factor: 1.843

4.  Transport and processing of staphylococcal alpha-toxin.

Authors:  R K Tweten; K K Christianson; J J Iandolo
Journal:  J Bacteriol       Date:  1983-11       Impact factor: 3.490

5.  Specific binding of staphylococcal alpha-toxin to isolated rabbit vagus nerves in vitro.

Authors:  S Szmigielski; S Harshman
Journal:  Infect Immun       Date:  1978-09       Impact factor: 3.441

6.  Inhibition of S. aureus alpha-hemolysin and B. anthracis lethal toxin by beta-cyclodextrin derivatives.

Authors:  Vladimir A Karginov; Ekaterina M Nestorovich; Frank Schmidtmann; Tanisha M Robinson; Adiamseged Yohannes; Nour Eddine Fahmi; Sergey M Bezrukov; Sidney M Hecht
Journal:  Bioorg Med Chem       Date:  2007-05-26       Impact factor: 3.641

7.  Induction of muscle-relaxing factor by staphylococcal alpha-toxin.

Authors:  S Harshman; N Sugg
Journal:  Infect Immun       Date:  1994-02       Impact factor: 3.441

8.  Site-directed mutagenesis of the alpha-toxin gene of Staphylococcus aureus: role of histidines in toxin activity in vitro and in a murine model.

Authors:  B E Menzies; D S Kernodle
Journal:  Infect Immun       Date:  1994-05       Impact factor: 3.441

9.  Toxicity of staphylococcal alpha toxin for rabbit alveolar macrophages.

Authors:  M P McGee; A Kreger; E S Leake; S Harshman
Journal:  Infect Immun       Date:  1983-01       Impact factor: 3.441

10.  The adjuvant activity of fatty acid esters. The role of acyl chain length and degree of saturation.

Authors:  R Bomford
Journal:  Immunology       Date:  1981-09       Impact factor: 7.397
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