Literature DB >> 861233

Studies on almond emulsin beta-D-glucosidase. I. Isolation and characterization of a bifunctional isozyme.

A K Grover, D D Macmurchie, R J Cushley.   

Abstract

A beta-D-glucosidase (beta-D-glucoside glucohydrolase, EC 3.2.1.21) isozyme has been isolated from almond emulsin. The isolated enzyme is a glycoprotein and migrates as a single band on Sephadex G-200 filtration, CM 52 ion exchange chromatography, polyacrylamide gel electrophoresis, sodium dodecyl sulfate polyacrylamide gel electrophoresis and isoelectric focussing. The glucosidase and galactosidase activities traverse together during Sephadex G-200 gel filtration. Polyacrylamide gels stained specifically for the 2 enzymes reveal that the two activities comigrate. The molecular weight of the isozyme has been found to be 135 180 +/- 770, and that of its protomers to be 65 150 +/- 650.

Entities:  

Mesh：

Substances：

Year:  1977        PMID： 861233     DOI： 10.1016/0005-2744(77)90358-8

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  9 in total

1.  Monitoring the active conformation of green fluorescent protein (GFP) and β-glucosidase adsorbed on soil particles.

Authors:  Isabel Morales-Belpaire; Patrick A Gerin
Journal:  Protein J       Date:  2012-01       Impact factor: 2.371

2.  Investigation of the slow inhibition of almond beta-glucosidase and yeast isomaltase by 1-azasugar inhibitors: evidence for the 'direct binding' model.

Authors:  A Lohse; T Hardlei; A Jensen; I W Plesner; M Bols
Journal:  Biochem J       Date:  2000-07-01       Impact factor: 3.857

3.  Cellulase recycling in high-solids enzymatic hydrolysis of pretreated empty fruit bunches.

Authors:  Jae Kyun Kim; Jungwoo Yang; So Young Park; Ju-Hyun Yu; Kyoung Heon Kim
Journal:  Biotechnol Biofuels       Date:  2019-06-06       Impact factor: 6.040

4.  Enhancing clot properties through fibrin-specific self-cross-linked PEG side-chain microgels.

Authors:  Nicole Welsch; Ashley C Brown; Thomas H Barker; L Andrew Lyon
Journal:  Colloids Surf B Biointerfaces       Date:  2018-03-02       Impact factor: 5.268

5.  Combined mass mapping and biochemical characterization of grape beta-glycosidase-enriched extract.

Authors:  Jean-Emmanuel Sarry; Jérôme Grimplet; Nicolas Sommerer; Marie-José Vallier; Martine Pradal; Laurence Mondolot; Claude Andary; Ziya Günata; Charles Romieu
Journal:  Protein J       Date:  2008-06       Impact factor: 2.371

6.  Arabinoamidine synthesis and its inhibition toward β-glucosidase (sweet almonds) in comparison to a library of galactonoamidines.

Authors:  Jessica B Pickens; Susanne Striegler; Qiu-Hua Fan
Journal:  Bioorg Med Chem       Date:  2016-05-07       Impact factor: 3.641

7.  Two New Native β-Glucosidases from Clavispora NRRL Y-50464 Confer Its Dual Function as Cellobiose Fermenting Ethanologenic Yeast.

Authors:  Xu Wang; Z Lewis Liu; Scott A Weber; Xiaoping Zhang
Journal:  PLoS One       Date:  2016-03-24       Impact factor: 3.240

8.  Expression of naturally ionic liquid-tolerant thermophilic cellulases in Aspergillus niger.

Authors:  Saori Amaike Campen; Jed Lynn; Stephanie J Sibert; Sneha Srikrishnan; Pallavi Phatale; Taya Feldman; Joel M Guenther; Jennifer Hiras; Yvette Thuy An Tran; Steven W Singer; Paul D Adams; Kenneth L Sale; Blake A Simmons; Scott E Baker; Jon K Magnuson; John M Gladden
Journal:  PLoS One       Date:  2017-12-27       Impact factor: 3.240

9.  Antidiabetic Activity and In Silico Molecular Docking of Polyphenols from Ammannia baccifera L. subsp. Aegyptiaca (Willd.) Koehne Waste: Structure Elucidation of Undescribed Acylated Flavonol Diglucoside.

Authors:  Noha Swilam; Mahmoud A M Nawwar; Rasha A Radwan; Eman S Mostafa
Journal:  Plants (Basel)       Date:  2022-02-06
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.