Literature DB >> 8603721

Measurement of nucleotide exchange kinetics with isolated synthetic myosin filaments using flash photolysis.

P B Conibear1, C R Bagshaw.   

Abstract

The kinetics of nucleotide release have been measured at the level of isolated synthetic myosin filaments. This was achieved by displacing a rhodamine nucleotide analog from a filament by flash photolysis of caged-ATP with selective observation using total internal reflectance fluorescence microscopy. The procedure gave improved time resolution over previously used flow methods. Kinetics were measured both in the presence of the ADP analog and during steady-state hydrolysis of the ATP analog. These studies open the way for kinetic measurements during actin filament sliding on myosin tracks.

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Year:  1996        PMID： 8603721     DOI： 10.1016/0014-5793(95)01538-8

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  10 in total

1.  Comparative single-molecule and ensemble myosin enzymology: sulfoindocyanine ATP and ADP derivatives.

Authors:  K Oiwa; J F Eccleston; M Anson; M Kikumoto; C T Davis; G P Reid; M A Ferenczi; J E Corrie; A Yamada; H Nakayama; D R Trentham
Journal:  Biophys J       Date:  2000-06       Impact factor: 4.033

2.  Role of actin cortex in the subplasmalemmal transport of secretory granules in PC-12 cells.

Authors:  T Lang; I Wacker; I Wunderlich; A Rohrbach; G Giese; T Soldati; W Almers
Journal:  Biophys J       Date:  2000-06       Impact factor: 4.033

Review 3.  Evanescent-wave microscopy: a new tool to gain insight into the control of transmitter release.

Authors:  M Oheim; D Loerke; R H Chow; W Stühmer
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  1999-02-28       Impact factor: 6.237

4.  Tracking single secretory granules in live chromaffin cells by evanescent-field fluorescence microscopy.

Authors:  J A Steyer; W Almers
Journal:  Biophys J       Date:  1999-04       Impact factor: 4.033

5.  Measurement of nucleotide exchange rate constants in single rabbit soleus myofibrils during shortening and lengthening using a fluorescent ATP analog.

Authors:  I Shirakawa; S Chaen; C R Bagshaw; H Sugi
Journal:  Biophys J       Date:  2000-02       Impact factor: 4.033

Review 6.  Engineering Dictyostelium discoideum myosin II for the introduction of site-specific fluorescence probes.

Authors:  Stuart Wakelin; Paul B Conibear; Robert J Woolley; David N Floyd; Clive R Bagshaw; Mihály Kovács; András Málnási-Csizmadia
Journal:  J Muscle Res Cell Motil       Date:  2002       Impact factor: 2.698

7.  Theory for ligand rebinding at cell membrane surfaces.

Authors:  B C Lagerholm; N L Thompson
Journal:  Biophys J       Date:  1998-03       Impact factor: 4.033

8.  Measurement of nucleotide release kinetics in single skeletal muscle myofibrils during isometric and isovelocity contractions using fluorescence microscopy.

Authors:  S Chaen; I Shirakawa; C R Bagshaw; H Sugi
Journal:  Biophys J       Date:  1997-10       Impact factor: 4.033

9.  Myosin regulatory light chain phosphorylation and strain modulate adenosine diphosphate release from smooth muscle Myosin.

Authors:  Alexander S Khromov; Martin R Webb; Michael A Ferenczi; David R Trentham; Andrew P Somlyo; Avril V Somlyo
Journal:  Biophys J       Date:  2004-04       Impact factor: 4.033

10.  Single turnovers of fluorescent ATP bound to bipolar myosin filament during actin filaments sliding.

Authors:  Takahiro Maruta; Takahiro Kobatake; Hiroyuki Okubo; Shigeru Chaen
Journal:  Biophysics (Nagoya-shi)       Date:  2013-01-19
  10 in total

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