| Literature DB >> 8597548 |
Y Nagasaka1, N Muraki, A Kimura, M Suto, A Yokota, F Tomita.
Abstract
A cDNA coding for the glucoamylase of Corticium rolfsii AHU 9627 was cloned using synthetic oligonucleotide probes that code for inner amino acid sequences of the purified enzyme. This clone (CG 15) is 1900 base pairs long and contains the entire coding region for a polypeptide of 579 residues. Comparison with amino acid sequences of other fungal glucoamylases showed homologies of 35%-56%, and most homology with that of Aspergillus niger. The expression plasmid pACG 115 was constructed by introduction of the coding region of CG 15 into a yeast expression vector pAAH 5, containing the promoter and terminator of alcohol dehydrogenase (ADHI). Saccharomyces cerevisiae AH 22, containing the recombinant plasmid pACG 115, acquired starch-saccharifying ability.Entities:
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Year: 1995 PMID： 8597548 DOI： 10.1007/bf00169943
Source DB: PubMed Journal: Appl Microbiol Biotechnol ISSN： 0175-7598 Impact factor: 4.813