Literature DB >> 8595856

Comparison of assays for Cryptosporidium parvum oocysts viability after chemical disinfection.

E K Black1, G R Finch, R Taghi-Kilani, M Belosevic.   

Abstract

In vitro excystation, vital dyes (4', 6-diamidino-2-phenylindole (DAPI) and propidium iodide (PI), and infectivity in neonatal CD-1 mice were used to assess the viability of Cryptosporidium parvum oocysts after chemical disinfection. In vitro excystation and DAPI/PI staining provided similar estimates of viability in bench-scale experiments, but both of these methods significantly overestimated the viability when compared with infectivity (Pr < or = 0.01). Infectivity was the most reliable measure of the viability of C. parvum oocysts following chemical disinfection.

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Year:  1996        PMID： 8595856     DOI： 10.1111/j.1574-6968.1996.tb07987.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  23 in total

1.  Comparison of animal infectivity and nucleic acid staining for assessment of Cryptosporidium parvum viability in water.

Authors:  N F Neumann; L L Gyürek; L Gammie; G R Finch; M Belosevic
Journal:  Appl Environ Microbiol       Date:  2000-01       Impact factor: 4.792

2.  Concentration and detection of cryptosporidium oocysts in surface water samples by method 1622 using ultrafiltration and capsule filtration.

Authors:  O D Simmons; M D Sobsey; C D Heaney; F W Schaefer; D S Francy
Journal:  Appl Environ Microbiol       Date:  2001-03       Impact factor: 4.792

3.  Use of a sentinel system for field measurements of Cryptosporidium parvum oocyst inactivation in soil and animal waste.

Authors:  M B Jenkins; M J Walker; D D Bowman; L C Anthony; W C Ghiorse
Journal:  Appl Environ Microbiol       Date:  1999-05       Impact factor: 4.792

4.  Assessment of a dye permeability assay for determination of inactivation rates of Cryptosporidium parvum oocysts.

Authors:  M B Jenkins; L J Anguish; D D Bowman; M J Walker; W C Ghiorse
Journal:  Appl Environ Microbiol       Date:  1997-10       Impact factor: 4.792

5.  Environmental temperature controls Cryptosporidium oocyst metabolic rate and associated retention of infectivity.

Authors:  Brendon J King; Alexandra R Keegan; Paul T Monis; Christopher P Saint
Journal:  Appl Environ Microbiol       Date:  2005-07       Impact factor: 4.792

Review 6.  Evaluation of the effect of temperature on the die-off rate for Cryptosporidium parvum oocysts in water, soils, and feces.

Authors:  X Peng; T Murphy; N M Holden
Journal:  Appl Environ Microbiol       Date:  2008-10-10       Impact factor: 4.792

7.  Viability of Cryptosporidium parvum oocysts: assessment by the dye permeability assay.

Authors:  L J Robertson; A T Campbell; H V Smith
Journal:  Appl Environ Microbiol       Date:  1998-09       Impact factor: 4.792

8.  Assessing the effects of tertiary treated wastewater reuse on a Mediterranean river (Llobregat, NE Spain): pathogens and indicators [corrected].

Authors:  María-Eugenia Rubiano; Míriam Agulló-Barceló; Raquel Casas-Mangas; Juan Jofre; Francisco Lucena
Journal:  Environ Sci Pollut Res Int       Date:  2011-07-01       Impact factor: 4.223

9.  Assessment of Giardia and Cryptosporidium spp. as a microbial source tracking tool for surface water: application in a mixed-use watershed.

Authors:  Natalie Prystajecky; Peter M Huck; Hans Schreier; Judith L Isaac-Renton
Journal:  Appl Environ Microbiol       Date:  2014-01-24       Impact factor: 4.792

10.  Comparison of Cryptosporidium parvum viability and infectivity assays following ozone treatment of oocysts.

Authors:  Z Bukhari; M M Marshall; D G Korich; C R Fricker; H V Smith; J Rosen; J L Clancy
Journal:  Appl Environ Microbiol       Date:  2000-07       Impact factor: 4.792
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