Literature DB >> 8590471

Detection of a protein which binds specifically to the upstream region of the pcbAB gene in Penicillium chrysogenum.

Y W Chu1, D Renno, G Saunders.   

Abstract

The upstream region of the pcbAB gene from Penicillium chrysogenum was screened for protein-binding sites using an electromobility shift assay. A specific protein/DNA interaction was detected within a fragment covering the region -387 to -242 relative to the pcbAB translational start codon. The appearance of this protein and pcbAB mRNA in culture extracts occurred at the same time point in fermentations, suggesting that the protein might be a transcription activator. The putative upstream activating sequence was located more precisely using cross-competition assays. These indicated the involvement of the 7-bp motif TGCCAAG in the binding of the protein.

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Year:  1995        PMID： 8590471     DOI： 10.1007/bf00315786

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  17 in total

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2.  Isolation and analysis of the Penicillium chrysogenum phoA gene encoding a secreted phosphate-repressible acid phosphatase.

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Journal:  Gene       Date:  1992-04-01       Impact factor: 3.688

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Authors:  C Bailey; H N Arst
Journal:  Eur J Biochem       Date:  1975-02-21

4.  Compilation of sequence-specific DNA-binding proteins implicated in transcriptional control in fungi.

Authors:  S S Dhawale; A C Lane
Journal:  Nucleic Acids Res       Date:  1993-12-11       Impact factor: 16.971

5.  Molecular characterization of a fungal secondary metabolism promoter: transcription of the Aspergillus nidulans isopenicillin N synthetase gene is modulated by upstream negative elements.

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Journal:  Mol Microbiol       Date:  1993-08       Impact factor: 3.501

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Authors:  E Montenegro; J L Barredo; S Gutiérrez; B Díez; E Alvarez; J F Martín
Journal:  Mol Gen Genet       Date:  1990-05

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Authors:  H Haas; E Friedlin; G Stöffler; B Redl
Journal:  Gene       Date:  1993-04-30       Impact factor: 3.688

8.  The multifunctional peptide synthetase performing the first step of penicillin biosynthesis in Penicillium chrysogenum is a 421,073 dalton protein similar to Bacillus brevis peptide antibiotic synthetases.

Authors:  D J Smith; A J Earl; G Turner
Journal:  EMBO J       Date:  1990-09       Impact factor: 11.598

9.  The Aspergillus nidulans npeA locus consists of three contiguous genes required for penicillin biosynthesis.

Authors:  A P MacCabe; M B Riach; S E Unkles; J R Kinghorn
Journal:  EMBO J       Date:  1990-01       Impact factor: 11.598

10.  pH regulation is a major determinant in expression of a fungal penicillin biosynthetic gene.

Authors:  E A Espeso; J Tilburn; H N Arst; M A Peñalva
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  7 in total

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Authors:  R Smidák; M Jopcík; M Kralovicová; J Gajdosíková; J Kormanec; J Timko; J Turna
Journal:  Folia Microbiol (Praha)       Date:  2010-05-19       Impact factor: 2.099

Review 3.  Molecular regulation of beta-lactam biosynthesis in filamentous fungi.

Authors:  A A Brakhage
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

4.  Binding of the PTA1 transcriptional activator to the divergent promoter region of the first two genes of the penicillin pathway in different Penicillium species.

Authors:  Katarina Kosalková; Marta Rodríguez-Sáiz; José Luis Barredo; Juan-Francisco Martín
Journal:  Curr Genet       Date:  2007-10-09       Impact factor: 3.886

5.  Identification of a major cis-acting DNA element controlling the bidirectionally transcribed penicillin biosynthesis genes acvA (pcbAB) and ipnA (pcbC) of Aspergillus nidulans.

Authors:  K T Bergh; O Litzka; A A Brakhage
Journal:  J Bacteriol       Date:  1996-07       Impact factor: 3.490

Review 6.  Hybrid antibiotics.

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Review 7.  Regulation and compartmentalization of β-lactam biosynthesis.

Authors:  Juan F Martín; Ricardo V Ullán; Carlos García-Estrada
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  7 in total

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