Literature DB >> 8586737

Frozen stored murine hybridoma cells can be used to determine the virulence of Listeria monocytogenes.

A K Bhunia1, D G Westbrook, R Story, M G Johnson.   

Abstract

Murine hybridoma cells, designated Ped-2E9, when stored up to 60 days at -196 degrees C or up to 48 days at -80 degrees C, gave results equivalent to those for freshly grown murine hybridoma cells in an in vitro pathogenicity assay of Listeria species. Thus, laboratories do not need to have their own tissue culture facilities to maintain the hybridoma cells for the assay described.

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Year:  1995        PMID: 8586737      PMCID: PMC228708          DOI: 10.1128/jcm.33.12.3349-3351.1995

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  8 in total

Review 1.  Epidemiology of human listeriosis.

Authors:  A Schuchat; B Swaminathan; C V Broome
Journal:  Clin Microbiol Rev       Date:  1991-04       Impact factor: 26.132

2.  In vitro model of penetration and intracellular growth of Listeria monocytogenes in the human enterocyte-like cell line Caco-2.

Authors:  J L Gaillard; P Berche; J Mounier; S Richard; P Sansonetti
Journal:  Infect Immun       Date:  1987-11       Impact factor: 3.441

3.  Monoclonal antibody-colony immunoblot method specific for isolation of Pediococcus acidilactici from foods and correlation with pediocin (bacteriocin) production.

Authors:  A K Bhunia; M G Johnson
Journal:  Appl Environ Microbiol       Date:  1992-07       Impact factor: 4.792

Review 4.  Listeria monocytogenes. A model system for the molecular study of intracellular parasitism.

Authors:  P Cossart; J Mengaud
Journal:  Mol Biol Med       Date:  1989-10

5.  Cytopathogenic effects in enterocytelike Caco-2 cells differentiate virulent from avirulent Listeria strains.

Authors:  L Pine; S Kathariou; F Quinn; V George; J D Wenger; R E Weaver
Journal:  J Clin Microbiol       Date:  1991-05       Impact factor: 5.948

6.  A six-hour in vitro virulence assay for Listeria monocytogenes using myeloma and hybridoma cells from murine and human sources.

Authors:  A K Bhunia; P J Steele; D G Westbrook; L A Bly; T P Maloney; M G Johnson
Journal:  Microb Pathog       Date:  1994-02       Impact factor: 3.738

Review 7.  Listeria monocytogenes, a food-borne pathogen.

Authors:  J M Farber; P I Peterkin
Journal:  Microbiol Rev       Date:  1991-09

8.  Pathogenicity test for Listeria monocytogenes using immunocompromised mice.

Authors:  G N Stelma; A L Reyes; J T Peeler; D W Francis; J M Hunt; P L Spaulding; C H Johnson; J Lovett
Journal:  J Clin Microbiol       Date:  1987-11       Impact factor: 5.948

  8 in total
  2 in total

1.  Rapid Ped-2E9 cell-based cytotoxicity analysis and genotyping of Bacillus species.

Authors:  Kristen M Gray; Padmapriya P Banada; Erin O'Neal; Arun K Bhunia
Journal:  J Clin Microbiol       Date:  2005-12       Impact factor: 5.948

2.  Mammalian Cell-Based Immunoassay for Detection of Viable Bacterial Pathogens.

Authors:  Luping Xu; Xingjian Bai; Shivendra Tenguria; Yi Liu; Rishi Drolia; Arun K Bhunia
Journal:  Front Microbiol       Date:  2020-11-23       Impact factor: 5.640

  2 in total

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