Literature DB >> 8586723

Purification of a baculovirus-expressed hepatitis E virus structural protein and utility in an enzyme-linked immunosorbent assay.

J He1, W M Ching, P Yarbough, H Wang, M Carl.   

Abstract

We report on the purification of the full-length structural protein encoded by open reading frame 2 (ORF-2) of hepatitis E virus. The ORF-2 protein, expressed in Sf9 cells by using a recombinant baculovirus vector system, was successfully purified to homogeneity. Gel electrophoresis of the purified ORF-2 protein showed a single polypeptide of 75 kDa by Coomassie blue staining and by Western blot (immunoblot) analysis. We demonstrated that the partially purified ORF-2 protein could be used successfully in a sensitive and specific enzyme-linked immunosorbent assay for the detection of antibodies to hepatitis E virus.

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Year:  1995        PMID： 8586723      PMCID： PMC228694          DOI： 10.1128/jcm.33.12.3308-3311.1995

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  21 in total

1.  Epidemiology.

Authors:  R VISWANATHAN
Journal:  Indian J Med Res       Date:  1957-01       Impact factor: 2.375

2.  Epidemic non-A, non-B hepatitis in patients from Pakistan.

Authors:  K M De Cock; D W Bradley; N L Sandford; S Govindarajan; J E Maynard; A G Redeker
Journal:  Ann Intern Med       Date:  1987-02       Impact factor: 25.391

3.  Epidemiological criteria as indication of non-A, non-B hepatitis in a community.

Authors: 
Journal:  Lancet       Date:  1985-10-12       Impact factor: 79.321

4.  Non-A, non-B epidemic hepatitis: visualization of virus-like particles in the stool by immune electron microscopy.

Authors:  M A Sreenivasan; V A Arankalle; A Sehgal; K M Pavri
Journal:  J Gen Virol       Date:  1984-05       Impact factor: 3.891

5.  Epidemic non-A, non-B hepatitis in Nepal. Recovery of a possible etiologic agent and transmission studies in marmosets.

Authors:  M A Kane; D W Bradley; S M Shrestha; J E Maynard; E H Cook; R P Mishra; D D Joshi
Journal:  JAMA       Date:  1984-12-14       Impact factor: 56.272

6.  Acute sporadic non-A, non-B hepatitis in India.

Authors:  M S Khuroo; W Duermeyer; S A Zargar; M A Ahanger; M A Shah
Journal:  Am J Epidemiol       Date:  1983-09       Impact factor: 4.897

7.  A clinical and epidemiological study of an epidemic of non-A non-B hepatitis in Rangoon.

Authors: 
Journal:  Am J Trop Med Hyg       Date:  1985-11       Impact factor: 2.345

8.  Expression and diagnostic utility of hepatitis E virus putative structural proteins expressed in insect cells.

Authors:  J He; A W Tam; P O Yarbough; G R Reyes; M Carl
Journal:  J Clin Microbiol       Date:  1993-08       Impact factor: 5.948

9.  Epidemic and endemic hepatitis in India: evidence for a non-A, non-B hepatitis virus aetiology.

Authors:  D C Wong; R H Purcell; M A Sreenivasan; S R Prasad; K M Pavri
Journal:  Lancet       Date:  1980-10-25       Impact factor: 79.321

10.  Evidence for a virus in non-A, non-B hepatitis transmitted via the fecal-oral route.

Authors:  M S Balayan; A G Andjaparidze; S S Savinskaya; E S Ketiladze; D M Braginsky; A P Savinov; V F Poleschuk
Journal:  Intervirology       Date:  1983       Impact factor: 1.763
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  2 in total

1.  Expression and self-assembly of empty virus-like particles of hepatitis E virus.

Authors:  T C Li; Y Yamakawa; K Suzuki; M Tatsumi; M A Razak; T Uchida; N Takeda; T Miyamura
Journal:  J Virol       Date:  1997-10       Impact factor: 5.103

Review 2.  Life cycle and morphogenesis of the hepatitis E virus.

Authors:  Kiyoshi Himmelsbach; Daniela Bender; Eberhard Hildt
Journal:  Emerg Microbes Infect       Date:  2018-11-29       Impact factor: 7.163
  2 in total

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