Literature DB >> 8579344

Superactivity of peroxidase solubilized in reversed micellar systems.

L Setti1, P Fevereiro, E P Melo, P G Pifferi, J M Cabral, M R Aires-Barros.   

Abstract

Vaccinium mirtyllus peroxidase solubilized in reversed micelles was used for the oxidation of guaiacol. Some relevant parameters for the enzymatic activity, such as pH, w(o) (molar ratio water/surfactant), surfactant type and concentration, and cosurfactant concentration, were investigated. The peroxidase showed higher activities in reversed micelles than in aqueous solution. The stability of the peroxidase in reversed micelles was also studied, namely, the effect of w(o) and temperature on enzyme deactivation. The peroxidase displayed higher stabilities in CTAB/hexanol in isooctane reversed micelles, with half-life times higher than 500 h.

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Year:  1995        PMID： 8579344     DOI： 10.1007/bf02786860

Source DB:  PubMed          Journal:  Appl Biochem Biotechnol        ISSN： 0273-2289            Impact factor:   2.926


  9 in total

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Journal:  Biochim Biophys Acta       Date:  1989-06-06

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Journal:  Eur J Biochem       Date:  1985-09-16

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Authors:  K Martinek; A V Levashov; Y L Khmelnitsky; N L Klyachko; I V Berezin
Journal:  Science       Date:  1982-11-26       Impact factor: 47.728

4.  [Micelle enzymology. The catalytic activity of peroxidase in a colloid solution of water in an organic solvent].

Authors:  K Martinek; N L Kliachko; A V Levashov; I V Berezin
Journal:  Dokl Akad Nauk SSSR       Date:  1983

5.  [Alcohol dehydrogenase substrate specificity in a colloidal solution of water in an organic solvent].

Authors:  K Martinek; Iu L Khmel'nitskiĭ; A V Levashov; I V Berezin
Journal:  Dokl Akad Nauk SSSR       Date:  1982

6.  The principles of enzyme stabilization. VI. Catalysis by water-soluble enzymes entrapped into reversed micelles of surfactants in organic solvents.

Authors:  K Martinek; A V Levashov; N L Klyachko; V I Pantin; I V Berezin
Journal:  Biochim Biophys Acta       Date:  1981-01-15

7.  How do organic solvents affect peroxidase structure and function?

Authors:  K Ryu; J S Dordick
Journal:  Biochemistry       Date:  1992-03-10       Impact factor: 3.162

8.  An unexpected side reaction in the guaiacol assay for peroxidase.

Authors:  A Taurog; M L Dorris; F S Guziec
Journal:  Anal Biochem       Date:  1992-09       Impact factor: 3.365

9.  Intestinal capillaries. I. Permeability to peroxidase and ferritin.

Authors:  F Clementi; G E Palade
Journal:  J Cell Biol       Date:  1969-04       Impact factor: 10.539
  9 in total
  2 in total

1.  Formation and properties of dimeric recombinant horseradish peroxidase in a system of reversed micelles.

Authors:  I G Gazaryan; N L Klyachko; Y K Dulkis; I V Ouporov; A V Levashov
Journal:  Biochem J       Date:  1997-12-01       Impact factor: 3.857

2.  Influence of different parameters on reverse micelle extraction combined with acetone precipitation to purify sn-1,3 extracellular lipase from Aspergillus niger GZUF36.

Authors:  Cuicui Chen; Hua Tian; Shuqi Xing; Cuiqin Li; Xuefeng Zeng; Laping He
Journal:  J Food Sci Technol       Date:  2019-05-06       Impact factor: 2.701
  2 in total

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