Literature DB >> 6753152

Colloidal solution of water in organic solvents: a microheterogeneous medium for enzymatic reactions.

K Martinek, A V Levashov, Y L Khmelnitsky, N L Klyachko, I V Berezin.   

Abstract

To simulate in vitro the conditions under which enzymes act in vivo, enzyme molecules have been entrapped in hydrated reverse micelles of a surfactant in organic solvents. In this system the catalytic activity of one of the enzymes studied (peroxidase) became much higher than in water, and the specificity of the other enzyme (alcohol dehydrogenase) was dramatically altered.

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Year:  1982        PMID: 6753152     DOI: 10.1126/science.6753152

Source DB:  PubMed          Journal:  Science        ISSN: 0036-8075            Impact factor:   47.728


  8 in total

1.  Superactivity and conformational changes on alpha-chymotrypsin upon interfacial binding to cationic micelles.

Authors:  M Soledad Celej; Mariana G D'Andrea; Patricia T Campana; Gerardo D Fidelio; M Lucia Bianconi
Journal:  Biochem J       Date:  2004-03-15       Impact factor: 3.857

2.  Studies on the immobilization of glucuronidase (Part 2). Cleavage of hardly soluble substrates in organic solvents.

Authors:  E Rapatz; M Ambros; B Kopp; F Pittner
Journal:  Appl Biochem Biotechnol       Date:  1988-12       Impact factor: 2.926

3.  Enzymes and cells in organic solvents and supercritical fluids. Patents and literature.

Authors:  R J Lindhardt
Journal:  Appl Biochem Biotechnol       Date:  1986-02       Impact factor: 2.926

Review 4.  Kinetic models in reverse micelles.

Authors:  R Bru; A Sánchez-Ferrer; F García-Carmona
Journal:  Biochem J       Date:  1995-09-15       Impact factor: 3.857

5.  Superactivity of peroxidase solubilized in reversed micellar systems.

Authors:  L Setti; P Fevereiro; E P Melo; P G Pifferi; J M Cabral; M R Aires-Barros
Journal:  Appl Biochem Biotechnol       Date:  1995-12       Impact factor: 2.926

6.  Modulation by n-alkanols of rat cardiac adenylate cyclase activity.

Authors:  P Chatelain; P Robberecht; M Waelbroeck; J C Camus; J Christophe
Journal:  J Membr Biol       Date:  1986       Impact factor: 1.843

7.  Lipase from Pseudomonas fragi CRDA 323: partial purification, characterization and interesterification of butter fat.

Authors:  F Pabai; S Kermasha; A Morin
Journal:  Appl Microbiol Biotechnol       Date:  1995-04       Impact factor: 4.813

8.  Triglyceride hydrolysis and stability of a recombinant cutinase from Fusarium solani in AOT-iso-octane reversed micelles.

Authors:  E P Melo; M R Aires-Barros; J M Cabral
Journal:  Appl Biochem Biotechnol       Date:  1995-01       Impact factor: 2.926

  8 in total

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