Agromyces mediolanus sp. nov., nom. rev., comb. nov., a species for "Corynebacterium mediolanum" Mamoli 1939 and for some aniline-assimilating bacteria which contain 2,4-diaminobutyric acid in the cell wall peptidoglycan.

In the course of identifying aniline-assimilating bacteria, researchers found some gram-positive strains that contain 2,4-diaminobutyric acid in their cell wall peptidoglycans and menaquinone 12 as the predominant menaquinone. "Corynebacterium mediolanum" and "Flavobacterium dehydrogenans" also are known to contain 2,4-diaminobutyric acid in their cell walls, as well as menaquinone 12, but the taxonomic position of these organisms has not been established previously. We found that the aniline-assimilating strains, together with "C. mediolanum" and "F. dehydrogenans," belong to a single species of the genus Agromyces, as determined by phenotypic characteristics, DNA-DNA relatedness data, and 16S ribosomal DNA sequence similarity data. The name Agromyces mediolanus sp. nov., nom. rev., comb. nov., is proposed for these organisms. The type strain of A. mediolanus is strain JCM 3346 (= ATCC 14004 = NCIMB 7206).