Literature DB >> 8571453

Finding prospective partners in the library: the two-hybrid system and phage display find a match.

J B Allen1, M W Walberg, M C Edwards, S J Elledge.   

Abstract

The two-hybrid system uses the efficacy of yeast genetic assays to identify protein-protein interactions. It permits the rapid cloning of genes encoding products that interact with a given protein of interest. Also being developed are phage display methods that allow direct physical selection of binding proteins. These methods have significantly altered strategies for analysing signaling and regulatory pathways.

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Year:  1995        PMID： 8571453     DOI： 10.1016/s0968-0004(00)89119-7

Source DB:  PubMed          Journal:  Trends Biochem Sci        ISSN： 0968-0004            Impact factor:   13.807


  31 in total

1.  A general strategy to enhance the potency of chimeric transcriptional activators.

Authors:  S Natesan; E Molinari; V M Rivera; R J Rickles; M Gilman
Journal:  Proc Natl Acad Sci U S A       Date:  1999-11-23       Impact factor: 11.205

2.  A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions.

Authors:  J K Joung; E I Ramm; C O Pabo
Journal:  Proc Natl Acad Sci U S A       Date:  2000-06-20       Impact factor: 11.205

3.  A green fluorescent protein-reporter mammalian two-hybrid system with extrachromosomal maintenance of a prey expression plasmid: application to interaction screening.

Authors:  T Shioda; S Andriole; T Yahata; K J Isselbacher
Journal:  Proc Natl Acad Sci U S A       Date:  2000-05-09       Impact factor: 11.205

4.  Physical and functional interaction of the Arabidopsis K(+) channel AKT2 and phosphatase AtPP2CA.

Authors:  Isabelle Chérel; Erwan Michard; Nadine Platet; Karine Mouline; Carine Alcon; Hervé Sentenac; Jean-Baptiste Thibaud
Journal:  Plant Cell       Date:  2002-05       Impact factor: 11.277

5.  Z-DNA-binding proteins can act as potent effectors of gene expression in vivo.

Authors:  Doo-Byoung Oh; Yang-Gyun Kim; Alexander Rich
Journal:  Proc Natl Acad Sci U S A       Date:  2002-12-16       Impact factor: 11.205

6.  Interaction of human apurinic endonuclease and DNA polymerase beta in the base excision repair pathway.

Authors:  R A Bennett; D M Wilson; D Wong; B Demple
Journal:  Proc Natl Acad Sci U S A       Date:  1997-07-08       Impact factor: 11.205

7.  Tetramerization of the AKT1 plant potassium channel involves its C-terminal cytoplasmic domain.

Authors:  P Daram; S Urbach; F Gaymard; H Sentenac; I Chérel
Journal:  EMBO J       Date:  1997-06-16       Impact factor: 11.598

8.  A tri-hybrid system for the analysis and detection of RNA--protein interactions.

Authors:  U Putz; P Skehel; D Kuhl
Journal:  Nucleic Acids Res       Date:  1996-12-01       Impact factor: 16.971

9.  Inhibition of parvovirus minute virus of mice replication by a peptide involved in the oligomerization of nonstructural protein NS1.

Authors:  A Pujol; L Deleu; J P Nüesch; C Cziepluch; J C Jauniaux; J Rommelaere
Journal:  J Virol       Date:  1997-10       Impact factor: 5.103

10.  Phage Therapy - Everything Old is New Again.

Authors:  Andrew M Kropinski
Journal:  Can J Infect Dis Med Microbiol       Date:  2006-09       Impact factor: 2.471
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