| Literature DB >> 856493 |
Abstract
A new method has been developed to improve the linearity of the plasma lecithin-cholesterol acyltransferase reaction by adding synthetic dipalmitoyl lecithin sol to the incubation medium and subsequently measuring the change in free cholesterol content after incubation by a combined enzymatic method using cholesterol oxidase and peroxidase. The method was found to be simpler than other techniques such as gas-liquid chromatography or isotope methods and therefore may be suitable for the routine assays of plasma lecithin-cholesterol acyltransferase activities in clinical laboratories.Entities:
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Year: 1977 PMID: 856493 DOI: 10.1016/0009-8981(77)90355-2
Source DB: PubMed Journal: Clin Chim Acta ISSN: 0009-8981 Impact factor: 3.786