Literature DB >> 8552626

Structure of the sodium channel pore revealed by serial cysteine mutagenesis.

M T Pérez-García1, N Chiamvimonvat, E Marban, G F Tomaselli.   

Abstract

The pores of voltage-gated cation channels are formed by four intramembrane segments that impart selectivity and conductance. Remarkably little is known about the higher order structure of these critical pore-lining or P segments. Serial cysteine mutagenesis reveals a pattern of side-chain accessibility that contradicts currently favored structural models based on alpha-helices or beta-strands. Like the active sites of many enzymes of known structure, the sodium channel pore consists of irregular loop regions.

Entities:  

Mesh：

Substances：

Year:  1996        PMID： 8552626      PMCID： PMC40226          DOI： 10.1073/pnas.93.1.300

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  33 in total

1.  Primary structure and functional expression of the human cardiac tetrodotoxin-insensitive voltage-dependent sodium channel.

Authors:  M E Gellens; A L George; L Q Chen; M Chahine; R Horn; R L Barchi; R G Kallen
Journal:  Proc Natl Acad Sci U S A       Date:  1992-01-15       Impact factor: 11.205

Review 2.  Pore loops: an emerging theme in ion channel structure.

Authors:  R MacKinnon
Journal:  Neuron       Date:  1995-05       Impact factor: 17.173

Review 3.  Structural basis of ion channel permeation and selectivity.

Authors:  W A Sather; J Yang; R W Tsien
Journal:  Curr Opin Neurobiol       Date:  1994-06       Impact factor: 6.627

4.  Primary structure of Electrophorus electricus sodium channel deduced from cDNA sequence.

Authors:  M Noda; S Shimizu; T Tanabe; T Takai; T Kayano; T Ikeda; H Takahashi; H Nakayama; Y Kanaoka; N Minamino
Journal:  Nature       Date:  1984 Nov 8-14       Impact factor: 49.962

5.  Side-chain accessibilities in the pore of a K+ channel probed by sulfhydryl-specific reagents after cysteine-scanning mutagenesis.

Authors:  L L Kürz; R D Zühlke; H J Zhang; R H Joho
Journal:  Biophys J       Date:  1995-03       Impact factor: 4.033

6.  Differential effects of sulfhydryl reagents on saxitoxin and tetrodotoxin block of voltage-dependent Na channels.

Authors:  G E Kirsch; M Alam; H A Hartmann
Journal:  Biophys J       Date:  1994-12       Impact factor: 4.033

7.  Molecular determinants of Ca2+ selectivity and ion permeation in L-type Ca2+ channels.

Authors:  J Yang; P T Ellinor; W A Sather; J F Zhang; R W Tsien
Journal:  Nature       Date:  1993-11-11       Impact factor: 49.962

8.  Silver as a probe of pore-forming residues in a potassium channel.

Authors:  Q Lü; C Miller
Journal:  Science       Date:  1995-04-14       Impact factor: 47.728

9.  A structural model of the tetrodotoxin and saxitoxin binding site of the Na+ channel.

Authors:  G M Lipkind; H A Fozzard
Journal:  Biophys J       Date:  1994-01       Impact factor: 4.033

10.  Identification of acetylcholine receptor channel-lining residues in the entire M2 segment of the alpha subunit.

Authors:  M H Akabas; C Kaufmann; P Archdeacon; A Karlin
Journal:  Neuron       Date:  1994-10       Impact factor: 17.173
View more
  47 in total

1.  Mechanisms of sodium/calcium selectivity in sodium channels probed by cysteine mutagenesis and sulfhydryl modification.

Authors:  M T Pérez-García; N Chiamvimonvat; R Ranjan; J R Balser; G F Tomaselli; E Marban
Journal:  Biophys J       Date:  1997-03       Impact factor: 4.033

2.  Novel interactions identified between micro -Conotoxin and the Na+ channel domain I P-loop: implications for toxin-pore binding geometry.

Authors:  Tian Xue; Irene L Ennis; Kazuki Sato; Robert J French; Ronald A Li
Journal:  Biophys J       Date:  2003-10       Impact factor: 4.033

3.  Constraint shapes convergence in tetrodotoxin-resistant sodium channels of snakes.

Authors:  Chris R Feldman; Edmund D Brodie; Edmund D Brodie; Michael E Pfrender
Journal:  Proc Natl Acad Sci U S A       Date:  2012-03-05       Impact factor: 11.205

4.  Ultra-slow inactivation in mu1 Na+ channels is produced by a structural rearrangement of the outer vestibule.

Authors:  H Todt; S C Dudley; J W Kyle; R J French; H A Fozzard
Journal:  Biophys J       Date:  1999-03       Impact factor: 4.033

5.  Adjacent pore-lining residues within sodium channels identified by paired cysteine mutagenesis.

Authors:  J P Bénitah; G F Tomaselli; E Marban
Journal:  Proc Natl Acad Sci U S A       Date:  1996-07-09       Impact factor: 11.205

6.  Chemical modification of the bacterial porin OmpF: gain of selectivity by volume reduction.

Authors:  Maarten Vrouenraets; Jenny Wierenga; Wim Meijberg; Henk Miedema
Journal:  Biophys J       Date:  2005-11-18       Impact factor: 4.033

7.  Transmembrane topology of a CLC chloride channel.

Authors:  T Schmidt-Rose; T J Jentsch
Journal:  Proc Natl Acad Sci U S A       Date:  1997-07-08       Impact factor: 11.205

8.  Calcium block of single sodium channels: role of a pore-lining aromatic residue.

Authors:  Vincent P Santarelli; Amy L Eastwood; Dennis A Dougherty; Christopher A Ahern; Richard Horn
Journal:  Biophys J       Date:  2007-06-01       Impact factor: 4.033

9.  Disulfide cross-linking of transport and trimerization domains of a neuronal glutamate transporter restricts the role of the substrate to the gating of the anion conductance.

Authors:  Mustafa Shabaneh; Noa Rosental; Baruch I Kanner
Journal:  J Biol Chem       Date:  2014-02-28       Impact factor: 5.157

10.  Differences in saxitoxin and tetrodotoxin binding revealed by mutagenesis of the Na+ channel outer vestibule.

Authors:  J L Penzotti; H A Fozzard; G M Lipkind; S C Dudley
Journal:  Biophys J       Date:  1998-12       Impact factor: 4.033
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.