A novel NF-kappa B p65 spliced transcript lacking exons 6 and 7 in a non-small cell lung carcinoma cell line.

Transcripts of the gene encoding the p65 subunit of the NF-kappa B/Rel transcription factor complex have been reported to undergo alternative splicing to generate one derivative lacking codons for amino acids (aa) 222 to 231 (p65 delta 1) and another that lacks codons for aa 13 to 25 (p65 delta 2) of the conserved Rel homology domain [Narayaran et al., Science 256 (1992) 317-320; Lyle et al., Gene 138 (1994) 265-266]. We have identified a third splicing event in a non-small-cell lung carcinoma cell line that potentially generates a novel p65 mRNA derivative lacking codons for aa 187 to 293 (p65 delta 3) of the Rel homology domain.