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Literature DB >> 853567

Synthesis of Newcastle disease virus polypeptides in a wheat germ cell-free system.

C W Clinkscales, M A Bratt, T G Morrison.

Abstract

We have isolated 18S RNA from cytoplasmic extracts of Newcastle disease virus-infected Chinese hamster ovary cells and tested its ability to direct protein synthesis in extracts derived from wheat germ. The products of the cell-free reaction directed by this RNA contain polypeptides that comigrate with NP, M,F, and 47K roteins from virions. In addition, the products contain a polypeptide (67K) that migrates on polyacrylamide gels slightly faster than the HN protein from virions. Tryptic peptide analysis of the cell-free products and proteins from virions confirms their identity.

Entities: Chemical Disease Species

Mesh：

Substances：

Year: 1977 PMID： 853567 PMCID： PMC515689 DOI： 10.1128/JVI.22.1.97-101.1977

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

21 in total

1. Site of synthesis of membrane and nonmembrane proteins of vesicular stomatitis virus.

Authors: T G Morrison
Journal: J Biol Chem Date: 1975-09-10 Impact factor: 5.157

2. Synthesis of Sendai virus polypeptides by a cell-free extract from wheat germ.

Authors: J W Davies; A Portner; D W Kingsbury
Journal: J Gen Virol Date: 1976-10 Impact factor: 3.891

3. Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity of proteolytic cleavage of an inactive precursor protein of Sendai virus.

Authors: A Scheid; P W Choppin
Journal: Virology Date: 1974-02 Impact factor: 3.616

4. Expression of animal virus genomes.

Authors: D Baltimore
Journal: Bacteriol Rev Date: 1971-09

5. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ.

Authors: B E Roberts; B M Paterson
Journal: Proc Natl Acad Sci U S A Date: 1973-08 Impact factor: 11.205

6. Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid.

Authors: R D Palmiter
Journal: Biochemistry Date: 1974-08-13 Impact factor: 3.162

3. Assembly of viral membranes: maturation of the vesicular stomatitis virus glycoprotein in the presence of tunicamycin.

Authors: T G Morrison; C O McQuain; D Simpson
Journal: J Virol Date: 1978-10 Impact factor: 5.103

4. Translation and membrane insertion of the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus.

Authors: C Wilson; R Gilmore; T Morrison
Journal: Mol Cell Biol Date: 1987-04 Impact factor: 4.272

5. Mutational changes in the vesicular stomatitis virus glycoprotein affect the requirement of carbohydrate in morphogenesis.

Authors: P A Chatis; T G Morrison
Journal: J Virol Date: 1981-01 Impact factor: 5.103

6. RNA synthesis by Newcastle disease virus temperature-sensitive mutants in two RNA-negative complementation groups.

Authors: M E Peeples; L L Rasenas; M A Bratt
Journal: J Virol Date: 1982-06 Impact factor: 5.103

7. Relationships among virus spread, cytopathogenicity, and virulence as revealed by the noncytopathic mutants of Newcastle disease virus.

Authors: C H Madansky; M A Bratt
Journal: J Virol Date: 1981-12 Impact factor: 5.103

Synthesis of Newcastle disease virus polypeptides in a wheat germ cell-free system.

1. Site of synthesis of membrane and nonmembrane proteins of vesicular stomatitis virus.

2. Synthesis of Sendai virus polypeptides by a cell-free extract from wheat germ.

3. Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity of proteolytic cleavage of an inactive precursor protein of Sendai virus.

4. Expression of animal virus genomes.

5. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ.

6. Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid.

7. Major human erythrocyte glycoprotein spans the cell membrane.

8. Polyadenylate sequences on Newcastle disease virus mRNA synthesized in vivo and in vitro.

9. Nucleocapsid protein subunits of simian virus 5, Newcastle disease virus, and Sendai virus.

10. Precursor protein for Newcastle disease virus.

1. Identification of Sendai virus mRNA species.

2. Assembly of viral membranes: nature of the association of vesicular stomatitis virus proteins to membranes.

3. Assembly of viral membranes: maturation of the vesicular stomatitis virus glycoprotein in the presence of tunicamycin.

4. Translation and membrane insertion of the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus.

5. Mutational changes in the vesicular stomatitis virus glycoprotein affect the requirement of carbohydrate in morphogenesis.

6. RNA synthesis by Newcastle disease virus temperature-sensitive mutants in two RNA-negative complementation groups.

7. Relationships among virus spread, cytopathogenicity, and virulence as revealed by the noncytopathic mutants of Newcastle disease virus.

8. Characterization of the soluble glycoprotein released from vesicular stomatitis virus-infected cells.

9. Synthesis, stability, and cleavage of Newcastle disease virus glycoproteins in the absence of glycosylation.

10. Structural and functional characterization of Newcastle disease virus polycistronic RNA species.