Literature DB >> 8532667

Engineering yeast alcohol dehydrogenase. Replacing Trp54 by Leu broadens substrate specificity.

E G Weinhold1, S A Benner.   

Abstract

Analysis of a crystal structure of alcohol dehydrogenase (Adh) from horse liver suggests that Trp54 in the homologous yeast alcohol dehydrogenase prevents the yeast enzyme from efficiently catalysing the oxidation of long-chain primary alcohols with branching at the 4 position (e.g. 4-methyl-1-pentanol, cinnamyl alcohol). This residue has been altered to Leu by site-directed mutagenesis. The alteration yields an enzyme that serves as an effective catalyst for both longer straight-chain primary alcohols and branched chain alcohols.

Entities:  

Mesh：

Substances：

Year:  1995        PMID： 8532667     DOI： 10.1093/protein/8.5.457

Source DB:  PubMed          Journal:  Protein Eng        ISSN： 0269-2139


  10 in total

1.  Purification and characterization of a novel NADP-dependent branched-chain alcohol dehydrogenase from Saccharomyces cerevisiae.

Authors:  M F van Iersel; M H Eppink; W J van Berkel; F M Rombouts; T Abee
Journal:  Appl Environ Microbiol       Date:  1997-10       Impact factor: 4.792

2.  Homogenates of yeast cultures with engineered catalases F148V and V111A reveal higher specific activities after incubation at permissive temperature.

Authors:  M Zámocký; F Koller
Journal:  Folia Microbiol (Praha)       Date:  1997       Impact factor: 2.099

3.  Structure-guided engineering of Lactococcus lactis alcohol dehydrogenase LlAdhA for improved conversion of isobutyraldehyde to isobutanol.

Authors:  Xiang Liu; Sabine Bastian; Christopher D Snow; Eric M Brustad; Tatyana E Saleski; Jian-He Xu; Peter Meinhold; Frances H Arnold
Journal:  J Biotechnol       Date:  2012-09-03       Impact factor: 3.307

4.  Mutations of gamma-aminobutyric acid and glycine receptors change alcohol cutoff: evidence for an alcohol receptor?

Authors:  M J Wick; S J Mihic; S Ueno; M P Mascia; J R Trudell; S J Brozowski; Q Ye; N L Harrison; R A Harris
Journal:  Proc Natl Acad Sci U S A       Date:  1998-05-26       Impact factor: 11.205

5.  Methionine 286 in transmembrane domain 3 of the GABAA receptor beta subunit controls a binding cavity for propofol and other alkylphenol general anesthetics.

Authors:  M D Krasowski; K Nishikawa; N Nikolaeva; A Lin; N L Harrison
Journal:  Neuropharmacology       Date:  2001-12       Impact factor: 5.250

6.  Molecular characterization of benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase II of Acinetobacter calcoaceticus.

Authors:  D J Gillooly; A G Robertson; C A Fewson
Journal:  Biochem J       Date:  1998-03-15       Impact factor: 3.857

7.  A discrete alcohol pocket involved in GIRK channel activation.

Authors:  Prafulla Aryal; Hay Dvir; Senyon Choe; Paul A Slesinger
Journal:  Nat Neurosci       Date:  2009-06-28       Impact factor: 24.884

Review 8.  Ethanol's molecular targets.

Authors:  R Adron Harris; James R Trudell; S John Mihic
Journal:  Sci Signal       Date:  2008-07-15       Impact factor: 8.192

9.  Yeast alcohol dehydrogenase structure and catalysis.

Authors:  Savarimuthu Baskar Raj; S Ramaswamy; Bryce V Plapp
Journal:  Biochemistry       Date:  2014-09-03       Impact factor: 3.162

10.  Development of a high-throughput assay for rapid screening of butanologenic strains.

Authors:  Chidozie Victor Agu; Stella M Lai; Victor Ujor; Pradip K Biswas; Andy Jones; Venkat Gopalan; Thaddeus Chukwuemeka Ezeji
Journal:  Sci Rep       Date:  2018-02-21       Impact factor: 4.379
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.