Literature DB >> 8521038

Preparation, manipulation, and pulse strategy for one-dimensional pulsed-field gel electrophoresis (ODPFGE).

J Noolandi1, C Turmel.   

Abstract

The underlying principles for zero-integrated-field electrophoresis (ZIFE) pulses and more general forward-biased pulse schemes are reviewed for one-dimensional pulsed-field gel electrophoresis (ODPFGE) separations of large DNA molecules. Detailed descriptions of materials, preparation protocols, hardware requirements, and procedures are given. A variety of gel pictures for known yeast DNA markers are shown.

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Year:  1995        PMID： 8521038     DOI： 10.1007/bf02907469

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  12 in total

1.  Field inversion gel electrophoresis with different pulse time ramps.

Authors:  C Heller; F M Pohl
Journal:  Nucleic Acids Res       Date:  1990-11-11       Impact factor: 16.971

2.  Molecular detrapping and band narrowing with high frequency modulation of pulsed field electrophoresis.

Authors:  C Turmel; E Brassard; G W Slater; J Noolandi
Journal:  Nucleic Acids Res       Date:  1990-02-11       Impact factor: 16.971

3.  Construction of the physical map for three loci in chromosome band 13q14: comparison to the genetic map.

Authors:  M J Higgins; C Turmel; J Noolandi; P E Neumann; M Lalande
Journal:  Proc Natl Acad Sci U S A       Date:  1990-05       Impact factor: 11.205

4.  Preparation and Separation of Intact Chromosomes of Vertebrates by One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE).

Authors:  J Noolandi; C Turmel
Journal:  Methods Mol Biol       Date:  1992

5.  Quantitative analysis of the three regimes of DNA electrophoresis in agarose gels.

Authors:  G W Slater; J Rousseau; J Noolandi; C Turmel; M Lalande
Journal:  Biopolymers       Date:  1988-03       Impact factor: 2.505

6.  Construction of a Not I restriction map of the fission yeast Schizosaccharomyces pombe genome.

Authors:  J B Fan; Y Chikashige; C L Smith; O Niwa; M Yanagida; C R Cantor
Journal:  Nucleic Acids Res       Date:  1989-04-11       Impact factor: 16.971

7.  Optimized conditions for pulsed field gel electrophoretic separations of DNA.

Authors:  B W Birren; E Lai; S M Clark; L Hood; M I Simon
Journal:  Nucleic Acids Res       Date:  1988-08-11       Impact factor: 16.971

8.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors:  A P Feinberg; B Vogelstein
Journal:  Anal Biochem       Date:  1983-07-01       Impact factor: 3.365

9.  Preparation, restriction, and hybridization analysis of Mammalian genomic DNA for pulsed-field gel electrophoresis.

Authors:  D P
Journal:  Methods Mol Biol       Date:  1992

10.  Comparison of the separation of Candida albicans chromosome-sized DNA by pulsed-field gel electrophoresis techniques.

Authors:  B A Lasker; G F Carle; G S Kobayashi; G Medoff
Journal:  Nucleic Acids Res       Date:  1989-05-25       Impact factor: 16.971
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