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Literature DB >> 8517758

Screening by polymerase chain reaction of Bacillus thuringiensis serotypes for the presence of cryV-like insecticidal protein genes and characterization of a cryV gene cloned from B. thuringiensis subsp. kurstaki.

Abstract

Polymerase chain reaction screening using cryV-specific oligonucleotides, designed to amplify the 5' half of cryV-type genes, revealed the presence of such genes in 7 of 21 Bacillus thuringiensis serotypes examined. Restriction analysis and hybridization studies indicated that these putative genes fall into at least three subclasses. The nucleotide sequence of the cryV-type gene cloned from B. thuringiensis subsp. kurstaki DSIR732 revealed an open reading frame coding for a protein of 719 amino acids, and lysates of Escherichia coli cells expressing the 81.2-kDa CryV732 protein were toxic to Epiphyas postvittana (Lepidoptera: Tortricidae).

Entities: Chemical Species

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Year: 1993 PMID： 8517758 PMCID： PMC182139 DOI： 10.1128/aem.59.5.1683-1687.1993

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

13 in total

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Authors: A P Gleave; R J Hedges; A H Broadwell
Journal: J Gen Microbiol Date: 1992-01

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Journal: Mol Microbiol Date: 1992-05 Impact factor: 3.501

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Journal: Nature Date: 1991-10-31 Impact factor: 49.962

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Authors: W H McGaughey
Journal: Science Date: 1985-07-12 Impact factor: 47.728

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Authors: H E Schnepf; K Tomczak; J P Ortega; H R Whiteley
Journal: J Biol Chem Date: 1990-12-05 Impact factor: 5.157

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Journal: J Bacteriol Date: 1991-07 Impact factor: 3.490

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Journal: J Bacteriol Date: 1990-12 Impact factor: 3.490

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Authors: F W Studier; B A Moffatt
Journal: J Mol Biol Date: 1986-05-05 Impact factor: 5.469

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Authors: J Van Rie; W H McGaughey; D E Johnson; B D Barnett; H Van Mellaert
Journal: Science Date: 1990-01-05 Impact factor: 47.728

10. DNA sequencing with chain-terminating inhibitors.

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Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

20 in total

1. Detection and identification of cry1I genes in Bacillus thuringiensis using polymerase chain reaction and restriction fragment length polymorphism analysis.

Authors: Diego H Sauka; Jorge G Cozzi; Graciela B Benintende
Journal: Curr Microbiol Date: 2005-12-26 Impact factor: 2.188

2. Structural analysis and molecular dynamics simulations of novel δ-endotoxin Cry1Id from Bacillus thuringiensis to pave the way for development of novel fusion proteins against insect pests of crops.

Authors: Budheswar Dehury; Mousumi Sahu; Jagajjit Sahu; Kishore Sarma; Priyabrata Sen; Mahendra K Modi; Madhumita Barooah; Manabendra Dutta Choudhury
Journal: J Mol Model Date: 2013-10-24 Impact factor: 1.810

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Authors: N Crickmore; D R Zeigler; J Feitelson; E Schnepf; J Van Rie; D Lereclus; J Baum; D H Dean
Journal: Microbiol Mol Biol Rev Date: 1998-09 Impact factor: 11.056

4. Molecular and insecticidal characterization of a Cry1I protein toxic to insects of the families Noctuidae, Tortricidae, Plutellidae, and Chrysomelidae.

Authors: Iñigo Ruiz de Escudero; Anna Estela; Manuel Porcar; Clara Martínez; José A Oguiza; Baltasar Escriche; Juan Ferré; Primitivo Caballero
Journal: Appl Environ Microbiol Date: 2006-07 Impact factor: 4.792

5. Biochemical and molecular characterization of the insecticidal fragment of CryV.

Authors: V Sekar; B Held; J Tippett; B Amirhusin; P Robeff; K Wang; H M Wilson
Journal: Appl Environ Microbiol Date: 1997-07 Impact factor: 4.792

6. Cloning of a cryV-type insecticidal protein gene from Bacillus thuringiensis: the cryV-encoded protein is expressed early in stationary phase.

Authors: K Kostichka; G W Warren; M Mullins; A D Mullins; N V Palekar; J A Craig; M G Koziel; J J Estruch
Journal: J Bacteriol Date: 1996-04 Impact factor: 3.490