| Literature DB >> 8513319 |
S F Nelson1, J H McCusker, M A Sander, Y Kee, P Modrich, P O Brown.
Abstract
Genomic mismatch scanning (GMS) is a new method of genetic linkage analysis that does not require conventional polymorphic markers or gel electrophoresis. GMS is ideally suited to affected-relative-pair mapping. DNA fragments from all regions of identity-by-descent between two relatives are isolated based on their ability to form extensive mismatch-free hybrid molecules. The genomic origin of this selected pool of DNA fragments is then mapped in a single hybridization step. Here we demonstrate the practicality of GMS in a model organism, Saccharomyces cerevisiae. GMS is likely to be applicable to other organisms, including humans, and may be of particular value in mapping complex genetic traits.Entities:
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Year: 1993 PMID： 8513319 DOI： 10.1038/ng0593-11
Source DB: PubMed Journal: Nat Genet ISSN： 1061-4036 Impact factor: 38.330