Literature DB >> 8510640

Heat-stable and heat-labile thymidylate synthases B of Bacillus subtilis: comparison of the nucleotide and amino acid sequences.

M Montorsi1, R Lorenzetti.   

Abstract

Bacillus subtilis 168 is known to possess two thymidylate synthase (TSase; EC 2.1.1.45) genes: thyA and thyB. thyB encodes a thermosensitive TSase (inactivated at 46 degrees C) which, in wild-type cells, accounts for only 5-8% of the total cellular TSase activity. In order to investigate the thermal lability of TSaseB we have analyzed the thyB genes of B. subtilis 168 and of an unrelated strain B. subtilis ATCC6633, which is shown here to have a temperature-resistant TSaseB. This conclusion is supported by the frequency of appearance of spontaneous Thy- mutants at 37 degrees C and 46 degrees C, and by the analysis of clones containing the thyB genes from the two strains. The nucleotide sequences of these two thyB genes were compared.

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Year:  1993        PMID： 8510640     DOI： 10.1007/BF00281594

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  21 in total

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Authors:  J L FARMER; F ROTHMAN
Journal:  J Bacteriol       Date:  1965-01       Impact factor: 3.490

2.  IMPROVED METHOD FOR THE ISOLATION OF THYMINE-REQUIRING MUTANTS OF ESCHERICHIA COLI.

Authors:  K A STACEY; E SIMSON
Journal:  J Bacteriol       Date:  1965-08       Impact factor: 3.490

3.  Crystal structure of Escherichia coli thymidylate synthase containing bound 5-fluoro-2'-deoxyuridylate and 10-propargyl-5,8-dideazafolate.

Authors:  D A Matthews; K Appelt; S J Oatley; N H Xuong
Journal:  J Mol Biol       Date:  1990-08-20       Impact factor: 5.469

4.  REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.

Authors:  C Anagnostopoulos; J Spizizen
Journal:  J Bacteriol       Date:  1961-05       Impact factor: 3.490

5.  Mutants of Escherichia coli requiring methionine or vitamin B12.

Authors:  B D DAVIS; E S MINGIOLI
Journal:  J Bacteriol       Date:  1950-07       Impact factor: 3.490

6.  Inhibition of peptide chain initiation in Escherichia coli by thermorubin.

Authors:  G Pirali; S Somma; G C Lancini; F Sala
Journal:  Biochim Biophys Acta       Date:  1974-10-28

7.  Two thymidylate synthetases in Bacillus subtilis.

Authors:  J Neuhard; A R Price; L Schack; E Thomassen
Journal:  Proc Natl Acad Sci U S A       Date:  1978-03       Impact factor: 11.205

8.  Thymidylate synthesis and aminopterin resistance in Bacillus subtilis.

Authors:  M C Wilson; J L Farmer; F Rothman
Journal:  J Bacteriol       Date:  1966-07       Impact factor: 3.490

9.  Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants.

Authors:  A M Friedman; S R Long; S E Brown; W J Buikema; F M Ausubel
Journal:  Gene       Date:  1982-06       Impact factor: 3.688

10.  Coregulation of dihydrofolate reductase and thymidylate synthase B in bacillus subtilis.

Authors:  T T Myoda; V L Funanage
Journal:  Biochim Biophys Acta       Date:  1985-02-20
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  1 in total

1.  From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later.

Authors:  Valérie Barbe; Stéphane Cruveiller; Frank Kunst; Patricia Lenoble; Guillaume Meurice; Agnieszka Sekowska; David Vallenet; Tingzhang Wang; Ivan Moszer; Claudine Médigue; Antoine Danchin
Journal:  Microbiology (Reading)       Date:  2009-04-21       Impact factor: 2.777
  1 in total

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