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Literature DB >> 418407

Two thymidylate synthetases in Bacillus subtilis.

J Neuhard, A R Price, L Schack, E Thomassen.

Abstract

Bacillus subtilis grown at temperatures below 37 degrees contains two thymidylate synthetases, TSaseA and TSaseB. Their presence is dependent on functional thyA and thyB genes, respectively. When cells are grown at 46 degrees they only contain TSaseA activity. This allous an easy positive selection for thyA and thyA, THYB mutants. The two TSases have been physically separated, and they show similar overall requirements for activity. However, they differ significantly in both their kinetic and their physicochemical properties.

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Year: 1978 PMID： 418407 PMCID： PMC411436 DOI： 10.1073/pnas.75.3.1194

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

17 in total

1. STUDIES ON LABILE DEOXYCYTIDYLATE HYDROXYMETHYLASES FROM ESCHERICHIA COLI B INFECTED WITH TEMPERATURE-SENSITIVE MUTANTS OF BACTERIOPHAGE T4.

Authors: J S WIBERG; J M BUCHANAN
Journal: Proc Natl Acad Sci U S A Date: 1964-03 Impact factor: 11.205

2. IMPROVED METHOD FOR THE ISOLATION OF THYMINE-REQUIRING MUTANTS OF ESCHERICHIA COLI.

Authors: K A STACEY; E SIMSON
Journal: J Bacteriol Date: 1965-08 Impact factor: 3.490

3. The enzymatic synthesis of thymidylate. I. Early steps in the purification of thymidylate synthetase of Escherichia coli.

Authors: A J WAHBA; M FRIEDKIN
Journal: J Biol Chem Date: 1962-12 Impact factor: 5.157

4. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.

Authors: C Anagnostopoulos; J Spizizen
Journal: J Bacteriol Date: 1961-05 Impact factor: 3.490

5. Altered deoxyribonucleotide pools in P2 eductants of Escherichia coli K-12 due to deletion of the dcd gene.

Authors: J Neuhard; E Thomassen
Journal: J Bacteriol Date: 1976-05 Impact factor: 3.490

6. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

7. Thymine utilization in Escherichia coli K12 on the role of deoxyribose 1-phosphate and thymidine phosphorylase.

Authors: K F Jensen; J C Leer; P Nygaard
Journal: Eur J Biochem Date: 1973-12-17

Review 8. Thymidylate synthetase.

Authors: M Friedkin
Journal: Adv Enzymol Relat Areas Mol Biol Date: 1973

Review 8. The Bacillus subtilis chromosome.

Authors: D J Henner; J A Hoch
Journal: Microbiol Rev Date: 1980-03

9. Specialized transduction of the ilvD-thyB-ilvA region mediated by Bacillus subtilis bacteriophage SP beta.

Authors: P S Fink; S A Zahler
Journal: J Bacteriol Date: 1982-06 Impact factor: 3.490

10. Bacillus subtilis glutamine synthetase mutants pleiotropically altered in glucose catabolite repression.

Authors: S H Fisher; A L Sonenshein
Journal: J Bacteriol Date: 1984-02 Impact factor: 3.490

Two thymidylate synthetases in Bacillus subtilis.

1. STUDIES ON LABILE DEOXYCYTIDYLATE HYDROXYMETHYLASES FROM ESCHERICHIA COLI B INFECTED WITH TEMPERATURE-SENSITIVE MUTANTS OF BACTERIOPHAGE T4.

2. IMPROVED METHOD FOR THE ISOLATION OF THYMINE-REQUIRING MUTANTS OF ESCHERICHIA COLI.

3. The enzymatic synthesis of thymidylate. I. Early steps in the purification of thymidylate synthetase of Escherichia coli.

4. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.

5. Altered deoxyribonucleotide pools in P2 eductants of Escherichia coli K-12 due to deletion of the dcd gene.

6. Protein measurement with the Folin phenol reagent.

7. Thymine utilization in Escherichia coli K12 on the role of deoxyribose 1-phosphate and thymidine phosphorylase.

Review 8. Thymidylate synthetase.

9. [Genetic determinism of the thymine requirement in certain Bacillus subtilis mutants].

10. Purine nucleoside phosphorylase from Escherichia coli and Salmonella typhimurium. Purification and some properties.

1. Selective association of the chromosome with membrane in a stable L-form of Bacillus subtilis.

Review 2. Revised genetic linkage map of Bacillus subtilis.

3. Deletion mutants of Bacillus subtilis bacteriophage SP beta.

4. RecD2 helicase limits replication fork stress in Bacillus subtilis.

5. Genetic analysis of autolysin-deficient and flagellaless mutants of Bacillus subtilis.

6. Crystal structure of thymidylate synthase A from Bacillus subtilis.

7. Attachment of the chromosomal terminus of Bacillus subtilis to a fast-sedimenting particle.

Review 8. The Bacillus subtilis chromosome.

9. Specialized transduction of the ilvD-thyB-ilvA region mediated by Bacillus subtilis bacteriophage SP beta.

10. Bacillus subtilis glutamine synthetase mutants pleiotropically altered in glucose catabolite repression.