Literature DB >> 8491805

Simultaneous determination of mevalonate and 7 alpha-hydroxycholesterol in human plasma by gas chromatography-mass spectrometry as indices of cholesterol and bile acid biosynthesis.

T Yoshida1, A Honda, N Tanaka, Y Matsuzaki, B He, T Osuga, N Kobayashi, K Ozawa, H Miyazaki.   

Abstract

A very sensitive and specific method for the simultaneous determination of mevalonate and 7 alpha-hydroxycholesterol in human plasma is described. The assay is based on isotope dilution mass spectrometry: the extracts from plasma were treated with benzylamine followed by dimethylethylsilylimidazole, then the resulting dimethylethylsilyl ether derivatives of mevalonylbenzylamide and 7 alpha-hydroxycholesterol were determined by gas chromatography-mass spectrometry using high-resolution selected-ion monitoring. Simple regression analysis revealed significant correlations between the plasma level of mevalonate and the hepatic activity of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (EC 1.1.1.34) (r = 0.83, P < 0.01) and between the plasma level of free 7 alpha-hydroxycholesterol and the hepatic activity of cholesterol 7 alpha-hydroxylase (EC 1.14.13.7) (r = 0.76, P < 0.05).

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Year:  1993        PMID： 8491805     DOI： 10.1016/0378-4347(93)80133-o

Source DB:  PubMed          Journal:  J Chromatogr


  10 in total

1.  Metabolomics: building on a century of biochemistry to guide human health.

Authors:  J Bruce German; Bruce D Hammock; Steven M Watkins
Journal:  Metabolomics       Date:  2005-03       Impact factor: 4.290

Review 2.  Review of progress in sterol oxidations: 1987-1995.

Authors:  L L Smith
Journal:  Lipids       Date:  1996-05       Impact factor: 1.880

3.  Short-term effects of 3-hydroxy-3-methylglutaryl-CoA reductase inhibitor on cholesterol and bile acid synthesis in humans.

Authors:  T Yoshida; A Honda; J Shoda; M Abei; Y Matsuzaki; N Tanaka; H Miyazaki; T Osuga
Journal:  Lipids       Date:  1997-08       Impact factor: 1.880

4.  Plasma levels of mevalonate and 7 alpha-hydroxy-4-cholestene-3-one in cholesterol gallstone disease and their etiological significance.

Authors:  J Shoda; B F He; N Tanaka; Y Matzuzaki; T Osuga
Journal:  J Gastroenterol       Date:  1994-02       Impact factor: 7.527

5.  An ultrasensitive enzymatic method for measuring mevalonic acid in serum.

Authors:  Takeshi Matsuoka; Shigeru Ueda; Hideyuki Matsumoto; Masanobu Kawakami
Journal:  J Lipid Res       Date:  2012-06-19       Impact factor: 5.922

6.  Regulation of cholesterol biosynthetic pathway in patients with the Smith-Lemli-Opitz syndrome.

Authors:  M Honda; G S Tint; A Honda; G Salen; S Shefer; A K Batta; Y Matsuzaki; N Tanaka
Journal:  J Inherit Metab Dis       Date:  2000-07       Impact factor: 4.982

7.  Alterations of bile acid composition in gallstones, bile, and liver of patients with hepatolithiasis, and their etiological significance.

Authors:  J Shoda; N Tanaka; B F He; Y Matsuzaki; T Osuga; H Miyazaki
Journal:  Dig Dis Sci       Date:  1993-11       Impact factor: 3.199

8.  Cholesterol and oxygenated cholesterol concentrations are markedly elevated in peripheral tissue but not in brain from mice with the Niemann-Pick type C phenotype.

Authors:  G S Tint; P Pentchev; G Xu; A K Batta; S Shefer; G Salen; A Honda
Journal:  J Inherit Metab Dis       Date:  1998-12       Impact factor: 4.982

9.  Increase of serum cholesterol levels by heat-moisture-treated high-amylose cornstarch in rats fed a high-cholesterol diet.

Authors:  Haruhide Udagawa; Chika Kitaoka; Tatsuaki Sakamoto; Kazuo Kobayashi-Hattori; Yuichi Oishi; Soichi Arai; Toshichika Takita
Journal:  Lipids       Date:  2008-05-29       Impact factor: 1.880

10.  Determination of key intermediates in cholesterol and bile acid biosynthesis by stable isotope dilution mass spectrometry.

Authors:  Tadashi Yoshida; Akira Honda; Hiroshi Miyazaki; Yasushi Matsuzaki
Journal:  Anal Chem Insights       Date:  2008-03-25
  10 in total

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